99mTc labeling white blood cells with a simple technique: clinical application

Several radionuclides and different methods have been employed as cellular labels to study inflammatory sites in man. Here we present the results obtained with white blood cells (WBC) labeled with 99mTc using a simple and low cost new technique (SnTec). WBC were incubated with 12 micrograms/ml of st...

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Bibliographic Details
Published in:Annals of nuclear medicine 1994-02, Vol.8 (1), p.85-89
Main Authors: Gutfilen, B, Pellini, M P, de Roure e Neder, J, de Amarante Júnior, J L, Evangelista, M G, Fernandes, S R, Bernardo-Filho, M
Format: Article
Language:English
Subjects:
Adolescent
Adult
Female
Humans
Isotope Labeling - methods
Leukocytes
Male
Middle Aged
Organotechnetium Compounds
Osteomyelitis - diagnostic imaging
Oximes
Radionuclide Imaging
Technetium
Technetium Tc 99m Exametazime
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Summary:Several radionuclides and different methods have been employed as cellular labels to study inflammatory sites in man. Here we present the results obtained with white blood cells (WBC) labeled with 99mTc using a simple and low cost new technique (SnTec). WBC were incubated with 12 micrograms/ml of stannous chloride for 10 min at room temperature. Then 99mTc was added. After 10 min, the 99mTc-labeled WBC were washed and injected into the patient. Comparison studies with 99mTc-labeled WBC using the HMPAO technique were carried out in patients with suspected osteomyelitis. Since the results are similar with both methods, we suggest the use of SnTec to label WBC, in cases of inflammatory diseases.
ISSN:0914-7187
1864-6433
DOI:10.1007/BF03164991