Ivermectin binding sites in sensitive and resistant Haemonchus contortus

Membranes from both ivermectin-sensitive and -resistant Haemonchus contortus L3larvae were examined for the presence of high affinity [3H]ivermectin binding sites. Both tissue preparations displayed high affinity drug binding sites (Kd= 0.13 nM). Receptor density (Bmax= 0.4 pmol/mg) was the same in...

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Bibliographic Details
Published in:The Journal of parasitology 1994-06, Vol.80 (3), p.493-497
Main Authors: Rohrer, S.P, Birzin, E.T, Eary, C.H, Schaeffer, J.M, Shoops, W.L
Format: Article
Language:English
Subjects:
Animals
Anthelmintics
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antiparasitic agents
Binding Sites
Biochemical mechanisms
Biological and medical sciences
CHEMICAL RESISTANCE
CHEMORECEPTORS
CHIMIORECEPTEUR
Culling
DENSIDAD
DENSITE
DENSITY
Drug resistance
Drug Resistance - physiology
DRUGS
Haemonchus - drug effects
Haemonchus - metabolism
HAEMONCHUS CONTORTUS
IVERMECTIN
Ivermectin - metabolism
Ivermectin - pharmacology
IVERMECTINA
IVERMECTINE
Larva - metabolism
Ligands
Medical sciences
MEDICAMENT
MEDICAMENTOS
Nematode larvae
Nematodes
Parasitology
Pharmacology. Drug treatments
QUIMIORECEPTORS
Receptors
Receptors, Drug - analysis
Receptors, Drug - physiology
Research Notes
RESISTANCE AUX PRODUITS CHIMIQUES
RESISTENCIA QUIMICA
SUSCEPTIBILITY
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Description
Summary:Membranes from both ivermectin-sensitive and -resistant Haemonchus contortus L3larvae were examined for the presence of high affinity [3H]ivermectin binding sites. Both tissue preparations displayed high affinity drug binding sites (Kd= 0.13 nM). Receptor density (Bmax= 0.4 pmol/mg) was the same in both the sensitive and resistant nematodes suggesting that target site modification was not involved in the development of drug resistance in this particular strain of H. contortus. The H. contortus ivermectin binding site appeared to be similar to the well characterized Caenorhabditis elegans ivermectin binding site with respect to affinity for ivermectin and receptor density.
ISSN:0022-3395
1937-2345
DOI:10.2307/3283426