Determination of Albendazole Metabolites in Plasma by HPLC

Albendazole is an antihelminthic agent belonging to the benzimidazole class and has been used successfully in the treatment of neurocysticercosis. We report here a method for the determination of the two major albendazole metabolites in plasma, albendazole sulfone and albendazole sulfoxide. The meth...

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Bibliographic Details
Published in:Journal of analytical toxicology 1994-03, Vol.18 (2), p.86-90
Main Authors: Valois, Maria Esther C., Takayanagui, Osvaldo Massaiti, Bonato, Pierina Sueli, Lanchote, Vera Lúcia, Carvalho, Dermeval
Format: Article
Language:English
Subjects:
Adolescent
Adult
Albendazole
Albendazole - analogs & derivatives
Albendazole - blood
Analysis
Benzimidazole
Biological and medical sciences
Child
Chromatography, High Pressure Liquid - methods
Cysticercosis
Drugs
Female
General pharmacology
High-performance liquid chromatography
Humans
Male
Mebendazole
Medical sciences
Metabolites
Middle Aged
n-Hexane
pH effects
Pharmacology. Drug treatments
Purification
Reproducibility of Results
sodium acetate
Taenia
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Summary:Albendazole is an antihelminthic agent belonging to the benzimidazole class and has been used successfully in the treatment of neurocysticercosis. We report here a method for the determination of the two major albendazole metabolites in plasma, albendazole sulfone and albendazole sulfoxide. The method consists of drug extraction from 500 µL of plasma previously acidified with chloroform-isopropanol (9:1, v/v) and extract purification with n-hexane immediately before chromatographic analysis. Separation of drugs and of the internal standard (mebendazole) was performed on an RP-18 column using acetonitrile-0.25N sodium acetate buffer (3:7, v/v), pH 5.0, as the mobile phase and using detection at 290 nm.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/18.2.86