The glpT and glpQ genes of the glycerol regulon in Bacillus subtilis

• Published in: Microbiology (Society for General Microbiology) 1994-04, Vol.140 (4), p.723-730
• Main Authors: Nilsson, Rune-Par, Beijer, Lena, Rutberg, Blanka
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Bacillus subtilis; Bacillus subtilis - genetics; Bacillus subtilis - metabolism; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; Base Sequence; Biological and medical sciences; Escherichia coli - genetics; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genetics; Glycerol - metabolism; Membrane Transport Proteins - genetics; Microbiology; Molecular Sequence Data; Nucleic Acid Conformation; Operon; Phosphoric Diester Hydrolases - genetics; Phosphoric Diester Hydrolases - metabolism; Repetitive Sequences, Nucleic Acid; Sequence Alignment; Sequence Homology, Amino Acid
• ISSN: 1350-0872; 1465-2080
• DOI: 10.1099/00221287-140-4-723

Department of Microbiology, Lund University, Sölvegatan 21, S-223 62 Lund, Sweden ABSTRACT The cloning of the Bacillus subtilis glpT and glpQ genes and their nucleotide sequences are reported. Analysis of mRNA indicates that glpT and glpQ constitute one operon which is transcribed from a sigma A type promoter. The steady state amount of glpTQ mRNA is increased in cells grown in the presence of glycerol 3-phosphate. The 5' untranslated leader sequence of glpTQ mRNA contains an inverted repeat which shows sequence similarity to repeats present in the leader sequences of glpFK and glpD transcripts. These repeats seem therefore to be essential control elements for all B. subtilis glp genes. 1 Author for correspondence: Rune-Pär Nilsson. Tel: [phone]. Fax [phone]. Keywords: Bacillus subtilis , glycerol regulon, glpT gene, glpQ gene