Human chromosomal centromere (AATGG) n sequence forms stable structures with unusual base pairs

Nine DNA sequences related to the purine strand of the human centromeric satellite (AATGG) n·(CCAAT) n repeat have been studied by two-dimensional nuclear magnetic resonance spectroscopy. Earlier studies have suggested that the structure of (AATGG) n sequence has an equilibrium between the duplex fo...

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Bibliographic Details
Published in:FEBS letters 1994-06, Vol.347 (1), p.99-103
Main Authors: Jaishree, T.N., Wang, Andrew H.-J.
Format: Article
Language:English
Subjects:
Base Composition
Base Sequence
Centromere
Centromere sequence
Chromosomes, Human
DNA structure
DNA, Satellite - chemistry
Humans
Magnetic Resonance Spectroscopy
Models, Molecular
Molecular Sequence Data
NMR
Nucleic Acid Conformation
Repetitive Sequences, Nucleic Acid
Unusual base pairs
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Summary:Nine DNA sequences related to the purine strand of the human centromeric satellite (AATGG) n·(CCAAT) n repeat have been studied by two-dimensional nuclear magnetic resonance spectroscopy. Earlier studies have suggested that the structure of (AATGG) n sequence has an equilibrium between the duplex form and a fold-back form. Structural refinement of d(CAATGG) and its related sequences by an NOE-constrained simulated annealing procedure reveals that the duplex form incorporates dynamic type-I G-A base pairs. 1D exchangeable proton NMR data support this model. The reverse sequence motif (GGTAA) destabilizes the structure.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(94)00516-8