Ultrastructural observations on the interaction in vitro between bovine eosinophils and juvenile Fasciola hepatica

This ultrastructural study has shown that there is a layer of dense flocculent material on the surface of juvenile Fasciola hepatica incubated in vitro with specific bovine antiserum. This material corresponds to the complexes of secreted glycocalyx and bovine antibody previously characterized by fl...

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Bibliographic Details
Published in:Parasitology 1985-12, Vol.91 (3), p.459-470
Main Authors: Glauert, A.M, Lammas, D.A, Duffus, W.P.H
Format: Article
Language:English
Subjects:
Animals
Antibodies - immunology
antigen-antibody complex
Antigen-Antibody Complex - immunology
Antigens, Helminth - immunology
antiserum
Biological and medical sciences
cattle
Cattle - immunology
Cattle - parasitology
Cell Membrane - ultrastructure
Diseases caused by trematodes
Distomatoses
eosinophils
Eosinophils - immunology
Eosinophils - ultrastructure
Fasciola hepatica
Fasciola hepatica - immunology
Fasciola hepatica - ultrastructure
Female
Glycoproteins
Helminthic diseases
Infectious diseases
integument
interactions
Medical sciences
Microscopy, Electron
Parasitic diseases
Polysaccharides
Tropical medicine
ultrastructure
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Summary:This ultrastructural study has shown that there is a layer of dense flocculent material on the surface of juvenile Fasciola hepatica incubated in vitro with specific bovine antiserum. This material corresponds to the complexes of secreted glycocalyx and bovine antibody previously characterized by fluorescence microscopy. Bovine eosinophils attach closely to those regions of the parasite's surface that are free of flocculent precipitates. This close attachment is followed by degranulation of the eosinophils into the narrow zone between the cells and the parasite. Only in these regions is damage, in the form of vacuolation of the tegument, seen within the juvenile F. hepatica. It is concluded that the inability of bovine eosinophils to kill juvenile F. hepatica in the presence of specific antiserum results from the presence of a protective layer, consisting of antigen/antibody complexes, on the parasite's surface.
ISSN:0031-1820
1469-8161
DOI:10.1017/S0031182000062703