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The use of chymotrypsin/galactose to treat spermatozoa bound with anti-sperm antibodies prior to intra-uterine insemination

Anti-sperm antibodies in semen have been associated with a decrease in fertility potential. The question arises as to whether intra-uterine insemination (IUI) can improve pregnancy rates by merely allowing earlier capacitation and close timing to ovulation, or whether certain treatments of the sperm...

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Published in:	Human reproduction (Oxford) 1994-03, Vol.9 (3), p.484-488
Main Authors:	Bollendorf, A., Check, J.H., Katsoff, D., Fedele, A.
Format:	Article
Language:	English
Subjects:	albumin anti-sperm antibodies Autoantibodies - analysis Autoantibodies - metabolism Biological and medical sciences Birth control chymotrypsin Chymotrypsin - pharmacology Female galactose Galactose - pharmacology Gynecology. Andrology. Obstetrics Humans Immunosorbent Techniques Infertility, Male - immunology Infertility, Male - therapy Insemination, Artificial intra-uterine insemination Male Medical sciences Microspheres Pregnancy pregnancy rates Prospective Studies Serum Albumin, Bovine - pharmacology Spermatozoa - drug effects Spermatozoa - immunology Spermatozoa - physiology Sterility. Assisted procreation
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cited_by	cdi_FETCH-LOGICAL-c302t-b4eefcef15644c25e07aa5244115d947de6e15e7b20053db2295a286bf5641ef3
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container_start_page	484
container_title	Human reproduction (Oxford)
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creator	Bollendorf, A. Check, J.H. Katsoff, D. Fedele, A.
description	Anti-sperm antibodies in semen have been associated with a decrease in fertility potential. The question arises as to whether intra-uterine insemination (IUI) can improve pregnancy rates by merely allowing earlier capacitation and close timing to ovulation, or whether certain treatments of the spermatozoa add extra benefit. The study presented herein was designed to compare IUI using Percoll density separation with an albumin treatment versus chymotrypsin/galactose treatment. Sixteen patients were evaluated where IUI was randomized between both sperm treatments. Pregnancy rates/cycle were 25% (eight of 32) with chymotrypsin/galactose-treated spermatozoa compared to only 3% (one of 33) cycles with albumin-treated spermatozoa (P < 0.01). Since it has been reported that the proportions of spermatozoa showing immunobead binding for specific antibodies after chymotrypsin/galactose treatment remain unchanged, the exact mechanism for improvement is unknown; possibly chymotrypsin/galactose interferes with the function of the antibodies
doi_str_mv	10.1093/oxfordjournals.humrep.a138532
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Obstetrics ; Humans ; Immunosorbent Techniques ; Infertility, Male - immunology ; Infertility, Male - therapy ; Insemination, Artificial ; intra-uterine insemination ; Male ; Medical sciences ; Microspheres ; Pregnancy ; pregnancy rates ; Prospective Studies ; Serum Albumin, Bovine - pharmacology ; Spermatozoa - drug effects ; Spermatozoa - immunology ; Spermatozoa - physiology ; Sterility. 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The question arises as to whether intra-uterine insemination (IUI) can improve pregnancy rates by merely allowing earlier capacitation and close timing to ovulation, or whether certain treatments of the spermatozoa add extra benefit. The study presented herein was designed to compare IUI using Percoll density separation with an albumin treatment versus chymotrypsin/galactose treatment. Sixteen patients were evaluated where IUI was randomized between both sperm treatments. Pregnancy rates/cycle were 25% (eight of 32) with chymotrypsin/galactose-treated spermatozoa compared to only 3% (one of 33) cycles with albumin-treated spermatozoa (P < 0.01). Since it has been reported that the proportions of spermatozoa showing immunobead binding for specific antibodies after chymotrypsin/galactose treatment remain unchanged, the exact mechanism for improvement is unknown; possibly chymotrypsin/galactose interferes with the function of the antibodies</description><subject>albumin</subject><subject>anti-sperm antibodies</subject><subject>Autoantibodies - analysis</subject><subject>Autoantibodies - metabolism</subject><subject>Biological and medical sciences</subject><subject>Birth control</subject><subject>chymotrypsin</subject><subject>Chymotrypsin - pharmacology</subject><subject>Female</subject><subject>galactose</subject><subject>Galactose - pharmacology</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Immunosorbent Techniques</subject><subject>Infertility, Male - immunology</subject><subject>Infertility, Male - therapy</subject><subject>Insemination, Artificial</subject><subject>intra-uterine insemination</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microspheres</subject><subject>Pregnancy</subject><subject>pregnancy rates</subject><subject>Prospective Studies</subject><subject>Serum Albumin, Bovine - pharmacology</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - immunology</subject><subject>Spermatozoa - physiology</subject><subject>Sterility. 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Andrology. Obstetrics</topic><topic>Humans</topic><topic>Immunosorbent Techniques</topic><topic>Infertility, Male - immunology</topic><topic>Infertility, Male - therapy</topic><topic>Insemination, Artificial</topic><topic>intra-uterine insemination</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microspheres</topic><topic>Pregnancy</topic><topic>pregnancy rates</topic><topic>Prospective Studies</topic><topic>Serum Albumin, Bovine - pharmacology</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - immunology</topic><topic>Spermatozoa - physiology</topic><topic>Sterility. 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source	Oxford University Press:Jisc Collections:Oxford Journal Archive: Access period 2024-2025
subjects	albumin anti-sperm antibodies Autoantibodies - analysis Autoantibodies - metabolism Biological and medical sciences Birth control chymotrypsin Chymotrypsin - pharmacology Female galactose Galactose - pharmacology Gynecology. Andrology. Obstetrics Humans Immunosorbent Techniques Infertility, Male - immunology Infertility, Male - therapy Insemination, Artificial intra-uterine insemination Male Medical sciences Microspheres Pregnancy pregnancy rates Prospective Studies Serum Albumin, Bovine - pharmacology Spermatozoa - drug effects Spermatozoa - immunology Spermatozoa - physiology Sterility. Assisted procreation
title	The use of chymotrypsin/galactose to treat spermatozoa bound with anti-sperm antibodies prior to intra-uterine insemination
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