Mouse oocytes derived from in vitro grown primary ovarian follicles are fertile

A model culture system has been developed whereby individual, primary ovarian mouse follicles can be grown in vitro to the Graafian stage in the normal physiological time course, and then ovulated in response to luteinizing hormone. We report here on the successful fertilization and subsequent embry...

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Bibliographic Details
Published in:Human reproduction (Oxford) 1994-03, Vol.9 (3), p.527-532
Main Authors: Spears, Norah, Boland, Nicola I., Murray, Alison A., Gosden, Roger G.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Birth control
culture
Culture Techniques
Embryo Transfer
Embryonic and Fetal Development
Epidermal Growth Factor - pharmacology
Female
fertilization
Fertilization in Vitro
Fetal Blood
follicle
Follicle Stimulating Hormone - pharmacology
Gynecology. Andrology. Obstetrics
Medical sciences
Mice
Mice, Inbred C3H
Mice, Inbred C57BL
mouse
oocyte
Oocytes - physiology
Ovarian Follicle - cytology
Ovarian Follicle - physiology
Pregnancy
Sterility. Assisted procreation
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Summary:A model culture system has been developed whereby individual, primary ovarian mouse follicles can be grown in vitro to the Graafian stage in the normal physiological time course, and then ovulated in response to luteinizing hormone. We report here on the successful fertilization and subsequent embryo development of the oocytes from such follicles. This is the first time that oocytes from in-vitro matured whole follicles have been fertilized and shown to produce viable offspring in host animals. The study demonstrates that the culture system mimics physiological conditions for normal follicle development.
ISSN:0268-1161
1460-2350
DOI:10.1093/oxfordjournals.humrep.a138539