Improvement of plasmid stability of recombinant Bacillus‐subtilis cells in continuous immobilized cultures

: The immobilization of recombinant Bacillus subtilis in K‐carrageenan gel beads has been performed in order to study the growth conditions inside the gel beads and to improve plasmid stability. Bacterial colonies showing high cell density were studied using scanning electron microscopy. A series of...

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Bibliographic Details
Published in:FEMS microbiology reviews 1994-05, Vol.14 (1), p.63-67
Main Authors: Castet, JoséCharles, Craynest, Muriel, Barbotin, Jean‐Noël, Truffaut, Nicole
Format: Article
Language:English
Subjects:
Bacillus subtilis
Bacillus subtilis - genetics
Bacillus subtilis - growth & development
Bacillus subtilis - ultrastructure
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Carrageenan
Continuous culture
Fundamental and applied biological sciences. Psychology
Gene Amplification
Genetics
Immobilization
Industrial Microbiology
Microscopy, Electron, Scanning
Mission oriented research
Plasmid stability
Plasmids
Recombinant Bacillus subtilis
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Summary:: The immobilization of recombinant Bacillus subtilis in K‐carrageenan gel beads has been performed in order to study the growth conditions inside the gel beads and to improve plasmid stability. Bacterial colonies showing high cell density were studied using scanning electron microscopy. A series of continuous cultures of free and immobilized B. subtilis MT119 (pHV1431, pIL252 and pIL252 Kpn) have been developed without selection pressure. In the free‐cell systems, it was found that a loss of plasmid vectors occurred after a short period. In contrast, in the immobilized cell systems, plasmid‐free segregants were not detected in any of the cases during the first 80 h of the culture.
ISSN:0168-6445
1574-6976
DOI:10.1111/j.1574-6976.1994.tb00074.x