Determination of local oxygen consumption rates in tumors

At any location in a respiring tissue, partial pressure of oxygen (PO2) is influenced by the local oxygen consumption rate. Consumption rates in vascular tumor tissues have previously been estimated for macroscopic regions. Using oxygen electrodes, we measured PO2 profiles across microregions (87 mi...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1994-07, Vol.54 (13), p.3333-3336
Main Authors: DEWHIRST, M. W, SECOMB, T. W, ONG, E. T, HSU, R, GROSS, J. F
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Medical sciences
Microcirculation
Models, Biological
Muscle
Neoplasms - blood supply
Neoplasms - physiopathology
Oxygen Consumption
Partial Pressure
Pharmacology. Drug treatments
Rats
Rats, Inbred F344
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Summary:At any location in a respiring tissue, partial pressure of oxygen (PO2) is influenced by the local oxygen consumption rate. Consumption rates in vascular tumor tissues have previously been estimated for macroscopic regions. Using oxygen electrodes, we measured PO2 profiles across microregions (87 microns to 286 microns wide) of tumors (R3230AC mammary adenocarcinoma) in a rat dorsal skin flap preparation and mapped adjacent microvessels. By comparing measured PO2 values with theoretical simulations, we deduced local consumption rates. Results for six profiles ranged from 0.83 to 2.22 cm3 O2/100 g/min. The mean (+/- SD) was 1.52 +/- 0.51 cm3 O2/100 g/min. This technique permits investigation of variations in consumption at a microregional level.
ISSN:0008-5472
1538-7445