The lower pathway operon for benzoate catabolism in biphenyl-utilizing Pseudomonas sp. strain IC and the nucleotide sequence of the bphE gene for catechol 2,3-dioxygenase

• Published in: Microbiology (Society for General Microbiology) 1994-03, Vol.140 (3), p.499-508
• Main Authors: Carrington, Bruce, Lowe, Andrea, Shaw, Linda E, Williams, Peter A
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Base Sequence; Benzoates - metabolism; Benzoic Acid; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; Biphenyl Compounds - metabolism; Catechol 2,3-Dioxygenase; Chromosome Mapping; Cloning, Molecular; Dioxygenases; DNA, Bacterial - genetics; Fundamental and applied biological sciences. Psychology; Gene Expression; Genes, Bacterial; Genetics; Mission oriented research; Molecular Sequence Data; Operon; Oxygenases - genetics; Pseudomonas; Pseudomonas - classification; Pseudomonas - genetics; Pseudomonas - metabolism; Species Specificity; Substrate Specificity
• ISSN: 1350-0872; 1465-2080
• DOI: 10.1099/00221287-140-3-499

School of Biological Sciences, University of Wales, Bangor, Gwynedd LL57 2UW, UK 5 Author for correspondence: Peter A. Williams. Tel: [phone]. Fax: [phone]. ABSTRACT Pseudomonas sp. strain IC is able to grow on biphenyl, 3-methylbiphenyl and 4-methylbiphenyl. These are converted to benzoate and the corresponding methylbenzoates. The lower pathway genes for the catabolism of the benzoates were cloned on a 22 kb Hin dIII fragment. Hybridization with gene-specific probes from the meta pathways of other catabolic plasmids showed that the gene order was identical to that of the operons carrying the same function from TOL plasmids. The nucleotide sequence of a 1241 bp region carrying the whole of the bphE gene (for a catechol 2,3-dioxygenase) and the 5' end of the downstream bphG gene (for 2-hydroxy semialdehyde dehydrogenase) was determined. Both genes showed a high degree of homology with genes encoding isofunctional proteins from other Pseudomonas strains. The upper pathway genes for the conversion of biphenyl to benzoate have also been cloned but no linkage with the lower pathway operon has been detected. Pseudomonas strain IC contains a large plasmid pWW110 (> 200 kb) and there are indications that this plasmid carries the bph genes. Keywords: Pseudomonas sp. strain IC, bph genes, benzoate catabolism, biphenyl catabolism, catechol 2,3-dioxygenase