Perfusion systems for hybridoma cells based on sedimentation in chambers and erlenmeyer flasks

: An inclined sedimentation chamber and a modified 250‐ml Erlenmeyer flask have been used as separation devices for perfusion fermentations with hybridoma cells. The maximum cell density is increased 2–16‐fold compared to batch fermentations when the separation units are used. When the sedimentation...

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Bibliographic Details
Published in:FEMS microbiology reviews 1994-05, Vol.14 (1), p.89-91
Main Authors: Mette, Karen, Lassen, Krieger, Emborg, Claus
Format: Article
Language:English
Subjects:
Animal cells
Animals
Antibodies, Monoclonal - biosynthesis
Antibody
Biological and medical sciences
Biotechnology
Cell Count
Cell‐specific production
Continuous fermentation
Countercurrent sedimentation chamber
Erlenmeyer flask
Establishment of new cell lines, improvement of cultural methods, mass cultures
Eukaryotic cell cultures
Fermentation
Fundamental and applied biological sciences. Psychology
Hiybridomas
Hybridomas - cytology
Hybridomas - immunology
Hybridomas - metabolism
Immunoglobulin G - biosynthesis
Maximum cell density
Methods. Procedures. Technologies
Mice
Perfusion
Perfusion - instrumentation
Perfusion - methods
Sedimentation
Stirred tank
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Summary:: An inclined sedimentation chamber and a modified 250‐ml Erlenmeyer flask have been used as separation devices for perfusion fermentations with hybridoma cells. The maximum cell density is increased 2–16‐fold compared to batch fermentations when the separation units are used. When the sedimentation chamber is used, IgG is continuously produced and the daily production is increased by a factor 3.7 compared to batch fermentation.
ISSN:0168-6445
1574-6976
DOI:10.1111/j.1574-6976.1994.tb00078.x