Radioiodination and preliminary in vivo investigation of the alkaloid cryptolepine

In this paper we report the successful labelling of cryptolepine with 131I using the chloramine T method. Quality control by ITLC of the labelled product showed 80% labelling but when the mixture was equilibrated with some amberlite (Cl −) anion resin to exchange the excess free iodide, the radioche...

Full description

Saved in:
Bibliographic Details
Published in:The International journal of applied radiation and isotopes 1985-12, Vol.36 (12), p.1003-1004
Main Authors: Salako, Q., Ablordeppey, S.Y.S., Dwuma-Badu, D., Thornback, J.R.
Format: Article
Language:English
Subjects:
Adrenergic alpha-Antagonists - metabolism
Alkaloids - isolation & purification
Alkaloids - metabolism
Animals
Indole Alkaloids
Indoles
Iodine Radioisotopes
Kinetics
Plants, Medicinal
Quinolines
Radioisotope Dilution Technique
Rats
Tissue Distribution
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this paper we report the successful labelling of cryptolepine with 131I using the chloramine T method. Quality control by ITLC of the labelled product showed 80% labelling but when the mixture was equilibrated with some amberlite (Cl −) anion resin to exchange the excess free iodide, the radiochemical purity was raised to >90%. Preliminary biodistribution in a rat did not show specific localisation of the tracer; rather, rapid clearance from the blood was indicated. It appears that its main excretory pathway is the hepatobiliary tract despite its relatively small molecular weight of 365. Also, despite i.v. administration, an appreciable 8% uptake was found in the stomach at 1 h post injection, indicating the process of enterohepatic reflux. These findings may have some bearings on the antimicrobial property of the plant.
ISSN:0020-708X
DOI:10.1016/0020-708X(85)90268-6