Distinguishing between duplex and hairpin forms of RNA by 15N 1H heteronuclear NMR

A general method is described for distinguishing RNA hairpins from RNA duplexes by application of two-dimensional filtered nuclear Overhauser enhancement spectra on a 1:1 mixture of unlabeled and 99% 15N-labeled molecules. The method is applied to the RNA dodecamer rGGCGCUUGCGUC which can form an in...

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Bibliographic Details
Published in:FEBS letters 1994-06, Vol.347 (2), p.261-264
Main Authors: Aboul-ela, Fareed, Nikonowicz, Edward P., Pardi, Arthur
Format: Article
Language:English
Subjects:
15N
Base Composition
Base Sequence
Heteronuclear NMR
Isotope
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Molecular Structure
Nucleic Acid Conformation
RNA - chemistry
RNA duplex
RNA hairpin
RNA structure
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Summary:A general method is described for distinguishing RNA hairpins from RNA duplexes by application of two-dimensional filtered nuclear Overhauser enhancement spectra on a 1:1 mixture of unlabeled and 99% 15N-labeled molecules. The method is applied to the RNA dodecamer rGGCGCUUGCGUC which can form an intramolecular hairpin under low salt conditions and a duplex in high salt. This procedure allows unambiguous identification of RNA hairpins or duplexes under the same conditions that are used in the NMR solution structure determination.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(94)00564-8