Molecular cloning and characterization of the ctpA gene encoding a carboxyl-terminal processing protease. Analysis of a spontaneous photosystem II-deficient mutant strain of the cyanobacterium Synechocystis sp. PCC 6803

• Published in: The Journal of biological chemistry 1994-07, Vol.269 (30), p.19354-19359
• Main Authors: SHESTAKOV, S. V, ANBUDURAI, P. R, STANBEKOVA, G. E, GADZHIEV, A, LIND, L. K, PAKRASI, H. B
• Format: Article
• Language: English
• Subjects: Algal Proteins; Amino Acid Sequence; Analytical, structural and metabolic biochemistry; Base Sequence; Biological and medical sciences; Biological Transport; Carboxypeptidases; Cyanobacteria - enzymology; Cyanobacteria - genetics; Electron Transport; Endopeptidases - genetics; Endopeptidases - metabolism; Enzymes and enzyme inhibitors; Escherichia coli; Freshwater; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; Genetic Complementation Test; Hydrolases; Molecular Sequence Data; Mutation; Photosynthesis - genetics; Photosynthetic Reaction Center Complex Proteins; Pigments, Biological - analysis; Proprotein Convertases; Protein Processing, Post-Translational - genetics; Protein Sorting Signals - genetics; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Synechocystis
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(17)32175-0

A nitrofurantoin enrichment technique was used to isolate a spontaneous photosynthesis-deficient mutant strain of the unicellular cyanobacterium Synechocystis sp. PCC 6803. This mutant, SK18, lacked any photosystem II (PSII) activity, but had normal photosystem I. The SK18 mutant strain could not be complemented with known genes encoding various structural proteins of PSII, but could be complemented with a recombinant plasmid pSL523 containing a 1.4-kilobase pair EcoRI fragment of the chromosomal DNA from wild-type Synechocystis 6803 cells. Determination of the nucleotide sequence of this DNA fragment revealed a previously unidentified open reading frame (ORF) encoding a 427-residue-long polypeptide. Hydrophobicity analysis of the amino acid sequence suggested that this protein is largely hydrophilic. A stretch of the first 31 amino-terminal residues of the polypeptide resembled a bacterial signal peptide and may be responsible for the translocation of this protein to the lumen space of the thylakoid membranes. The spontaneous mutation in the SK18 strain was identified to be a single nucleotide change introducing a premature termination codon in this ORF. The predicted sequence of the encoded protein showed significant similarity to that of the Prc protein, a carboxyl-terminal processing protease in Escherichia coli. We suggest that the cyanobacterial protein encoded by ORF427 is a similar processing protease and name the gene ctpA (carboxyl-terminal processing protease).