Viral Replication and Development of Specific Immunity in Macaques after Infection with Different Measles Virus Strains

• Published in: The Journal of infectious diseases 1994-08, Vol.170 (2), p.443-448
• Main Authors: van Binnendijk, Robert S., van der Heijden, Roger W. J., Amerongen, Geert van, UytdeHaag, Fons G. C. M., Osterhaus, Albert D. M. E.
• Format: Article
• Language: English
• Subjects: Animals; Antibodies; Antibodies, Viral - biosynthesis; Concise Communications; Disease Models, Animal; Humans; Immunity, Cellular; Immunoglobulin G - biosynthesis; Immunoglobulin M - biosynthesis; Infections; Kinetics; Leukocytes, Mononuclear - microbiology; Lung - cytology; Lung - microbiology; Macaca fascicularis; Measles; Measles - immunology; Measles - microbiology; Measles virus; Measles virus - immunology; Measles virus - physiology; Monkeys; Pharynx - cytology; Pharynx - microbiology; T lymphocytes; T-Lymphocytes - immunology; Vaccination; Vero cells; Viremia; Viremia - immunology; Viremia - microbiology; Virus Replication; Viruses
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1093/infdis/170.2.443

Cynomolgus monkeys (Macaca fascicularis) were experimentally infected with a wild type measles virus (MY) strain (MY-BIL). Following intratracheal inoculation with different infectious doses, the virus could be isolated from peripheral blood mononuclear cells (PBMC), lung lavage cells, and pharyngeal cells. The kinetics of the cell-associated viremia was similar in all infected animals. They developed specific serum IgM, IgG, and neutralizing antibody responses as well as MY-specific T cell-mediated immunity. Monkeys infected intratracheally or intramuscularly with the wild type MY-Edmonston or the attenuated MY-Schwartz strain showed a lower level of PBMC-associated viremia and less pronounced specific IgM responses. Nine months after infection with MY strains, all of the monkeys were protected from intratracheal reinfection with MY-BIL. This monkey model is suitable for study of new generations of vaccines and vaccination strategies for measles.