In vitro ethanol effects on the transport properties of isolated renal brush-border membrane vesicles

The in vitro effect of ethanol on membrane structure and transport properties was studied in isolated renal brush border membrane vesicles. 31P-NMR studies showed a dose-dependent increase in the quantity of an isotropic, possibly inverted-micellar component of the renal brush-border membrane as a r...

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Bibliographic Details
Published in:The Journal of membrane biology 1985, Vol.88 (2), p.123-130
Main Authors: ELGAVISH, A, ELGAVISH, G. A
Format: Article
Language:English
Subjects:
Alcoholism and acute alcohol poisoning
Animals
Biological and medical sciences
Biological Transport, Active - drug effects
brush border membranes
Cell Membrane - metabolism
Dose-Response Relationship, Drug
ethanol
Ethanol - pharmacology
kidney
Kidney Cortex - metabolism
Liposomes - metabolism
Magnetic Resonance Spectroscopy
Medical sciences
Membrane Potentials - drug effects
Microvilli - drug effects
Microvilli - metabolism
Rats
Rats, Inbred Strains
sodium
Sodium - metabolism
Time Factors
Toxicology
vesicles
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Summary:The in vitro effect of ethanol on membrane structure and transport properties was studied in isolated renal brush border membrane vesicles. 31P-NMR studies showed a dose-dependent increase in the quantity of an isotropic, possibly inverted-micellar component of the renal brush-border membrane as a result of treatment with ethanol. Such structures have been shown to be instrumental in the translocation of material across membrane bilayers. A 23Na-NMR study of Na+ exchange in artificial phosphatidylcholine liposomes indicated that ethanol (0.1%) was capable of rendering the otherwise inert vesicles permeable to sodium, supporting the idea that ethanol may exert its action via a direct effect on the structure of the phospholipid bilayer. In the isolated renal brush-border membrane vesicles, like in the artificial liposomes, amiloride-insensitive pathways of Na+ transport were shown to be markedly activated by ethanol. These results were consistent with the inhibitory effect ethanol had on Na+ gradient-dependent transport systems such as the Na+ gradient-dependent D-glucose transport and Na+/H+ exchange. In conclusion, our results indicate that ethanol exerts its effect on the renal brush-border membrane by causing a structural change in the phospholipid bilayer which activates sodium intake. The inhibitory effect of ethanol on glucose uptake and Na+/H+ exchange is secondary, as a result of the dissipation of the energy-producing Na+ gradient.
ISSN:0022-2631
1432-1424
DOI:10.1007/BF01868426