Naturally occurring porcine relaxins and large-scale preparation of the B29 hormone

Porcine ovaries were collected from pregnant sows under conditions designed to keep autolysis to an absolute minimum. During the extraction the tissues were never allowed to warm up to 0 degree C until submerged in 1.6 N HCl. Isolation and fractionation of the various relaxin forms became possible b...

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Bibliographic Details
Published in:Biochemistry (Easton) 1985-12, Vol.24 (26), p.7717-7722
Main Authors: Bullesbach, Erika E, Schwabe, Christian
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Chromatography, High Pressure Liquid
Female
Fundamental and applied biological sciences. Psychology
Ovary - analysis
Pregnancy
Protein hormones. Growth factors. Cytokines
Proteins
Relaxin - isolation & purification
Swine
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Summary:Porcine ovaries were collected from pregnant sows under conditions designed to keep autolysis to an absolute minimum. During the extraction the tissues were never allowed to warm up to 0 degree C until submerged in 1.6 N HCl. Isolation and fractionation of the various relaxin forms became possible by application of CM-cellulose chromatography at pH 5.5 and 7.8, gel filtration, and high-performance liquid chromatography. The new isolation procedure has made it possible to isolate and identify LeuB32 relaxin. Also, [Leu-PheA0]B29 relaxin was identified and the existence of a [Leu-PheA0]B32 relaxin may be deduced from our data. Controlled digestion of B-chain-extended relaxins with carboxypeptidase A led to the large-scale production of homogeneous B29 relaxin, a suitable starting material for controlled chemical modification of porcine relaxin.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi00347a032