Effects of Pioglitazone on Calcium Channels in Vascular Smooth Muscle

Pioglitazone, an insulin-sensitizing, antidiabetic agent, has blood pressure-lowering effects in insulin-resistant hypertensive rats and attenuates growth factor-induced increases of intracellular Ca in rat aortic vascular smooth muscle cells. To determine whether modulation of voltagedependent Ca c...

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Bibliographic Details
Published in:Hypertension (Dallas, Tex. 1979) Tex. 1979), 1994-08, Vol.24 (2), p.170-175
Main Authors: Zhang, Feng, Sowers, James R, Ram, Jeffrey L, Standley, Paul R, Peuler, Jacob D
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Calcium Channels - drug effects
Cells, Cultured
Dimethyl Sulfoxide - pharmacology
General and cellular metabolism. Vitamins
Hypoglycemic Agents - pharmacology
Male
Medical sciences
Muscle, Smooth, Vascular - drug effects
Pharmacology. Drug treatments
Pioglitazone
Rats
Rats, Sprague-Dawley
Thiazoles - pharmacology
Thiazolidinediones
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Summary:Pioglitazone, an insulin-sensitizing, antidiabetic agent, has blood pressure-lowering effects in insulin-resistant hypertensive rats and attenuates growth factor-induced increases of intracellular Ca in rat aortic vascular smooth muscle cells. To determine whether modulation of voltagedependent Ca channels plays a role in this association, we investigated the effects of pioglitazone on voltage-dependent current in cultured rat aortic (a7r5) and freshly dissociated rat tail artery vascular smooth muscle cells. Both cell types were studied with whole-cell patch-clamp techniques. Current through L-type Ca channels was elicited with a voltage ramp in the presence of Ba substituted for Ca. T-type Ca current was studied using a two-pulse protocol that enabled the isolation of transient current. In a7r5 vascular smooth muscle cells, 2-minute application of pioglitazone (5 and 10 μmol/L) reduced L-type current by 7.9±1.0% (n=8) (mean±SEM, number of cells) and 14.5±3.0% (n=9) (P
ISSN:0194-911X
1524-4563
DOI:10.1161/01.hyp.24.2.170