A Method for the High Efficiency of Water-Soluble Carbodiimide-Mediated Amidation

Water-soluble carbodiimides are widely used for carboxyl-amine conjugation. However, extremely variable and low yields, obtained under a variety of conditions, have been a serious problem in the coupling. A simple method, optimizing various parameters of the coupling reaction, in which N-hydroxysucc...

Full description

Saved in:
Bibliographic Details
Published in:Analytical biochemistry 1994-04, Vol.218 (1), p.87-91
Main Authors: Sehgal, D., Vijay, I.K.
Format: Article
Language:English
Subjects:
alpha-Glucosidases - isolation & purification
Amides - chemistry
Animals
Biological and medical sciences
Catalysis
Ethyldimethylaminopropyl Carbodiimide - chemistry
Female
Fundamental and applied biological sciences. Psychology
General aspects
Instrumentation. Materials. Reagents. Research laboratory organization
Mammary Glands, Animal - enzymology
Molecular Structure
Rats
Solubility
Water - chemistry
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Water-soluble carbodiimides are widely used for carboxyl-amine conjugation. However, extremely variable and low yields, obtained under a variety of conditions, have been a serious problem in the coupling. A simple method, optimizing various parameters of the coupling reaction, in which N-hydroxysuccinimide is included to assist the 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride-catalyzed amidation reaction is described. A product yield of up to 90% is routinely achieved.
ISSN:0003-2697
1096-0309
DOI:10.1006/abio.1994.1144