Evidence for a trans-acting activator function regulating the expression of the human CD5 antigen

Interspecies somatic cell hybrids were generated by fusing the mouse T-lymphoma cell line, BW5147, with normal human T lymphocytes at different stages of differentiation. Thymocytes, activated peripheral T lymphocytes, or an activated T-cell clone were used as human partners, respectively, in three...

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Bibliographic Details
Published in:Immunogenetics (New York) 1994-07, Vol.40 (3), p.217-221
Main Authors: SCUPOLI, M. T, SARTORIS, S, NICOLIS, M, CESTARI, T, CAMBIAGGI, C, TRIDENTE, G, ACCOLLA, R. S
Format: Article
Language:English
Subjects:
Animals
Antigens, CD - genetics
Biological and medical sciences
CD5 Antigens
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Expression Regulation
Humans
Hybrid Cells
Molecular and cellular biology
Molecular genetics
Rats
T-Lymphocytes - immunology
Trans-Activators
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Summary:Interspecies somatic cell hybrids were generated by fusing the mouse T-lymphoma cell line, BW5147, with normal human T lymphocytes at different stages of differentiation. Thymocytes, activated peripheral T lymphocytes, or an activated T-cell clone were used as human partners, respectively, in three independent fusions. Irrespective of the human cell partner used for fusion, a certain number of hybrids lost CD5 surface expression over a period of time in culture. Analysis at the phenotype and genetic level showed that lack of CD5 expression was due neither to segregation of human autosome 11, on which the CD5 gene has been mapped, nor to deletion of the CD5 structural gene. Furthermore, loss of CD5 surface expression correlated with the absence of specific mRNA. Since these hybrids preferentially segregate human chromosomes, these results indicate the existence of a non-syntenic trans-active locus, or loci, positively controlling the expression of the human CD5 gene.
ISSN:0093-7711
1432-1211
DOI:10.1007/BF00167082