Lactate transport in rat sarcolemmal vesicles and intact skeletal muscle, and after muscle contraction

• Published in: Acta physiologica Scandinavica 1994-05, Vol.151 (1), p.17-28
• Main Authors: McDERMOTT, J. C., BONEN, A.
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Biological Transport; Cell Membrane - metabolism; Cell Membrane Permeability; electrical stimulation; Fundamental and applied biological sciences. Psychology; glucose; Glucose - metabolism; Hydrogen-Ion Concentration; In Vitro Techniques; Kinetics; lactate; Lactates - metabolism; Male; Muscle Contraction; Muscles - metabolism; plasma membranes; Rats; Rats, Sprague-Dawley; Sarcolemma - metabolism; Striated muscle. Tendons; transport; Vertebrates: osteoarticular system, musculoskeletal system
• ISSN: 0001-6772; 1365-201X
• DOI: 10.1111/j.1748-1716.1994.tb09717.x

To determine whether it was possible to measure lactate transport rates into intact skeletal muscles, the transport of lactate (zero‐trans) was determined in soleus muscle strips incubated in vitro and compared with lactate transport in sarcolemmal vesicles. In addition, the effects of muscle contractility on lactate transport were investigated in electrically‐stimulated soleus muscle strips. In both the intact muscle and the sarcolemmal preparations the rates of transport were saturable, stereospecific, and inhibitable by monocarboxylates (pyruvate, α‐cyano‐4‐hydroxycinnamate) and a protein modifier (A'‐ethylmaleimide; P < 0.05). The anion exchange inhibitor SITS had no effect on lactate uptake (P > 0.05). In both preparations lactate transport followed an inwardly directed proton gradient. Relative comparisons (%) between the preparations indicated a similar slope of increasing transport rates with increasing lactate concentrations and similar responses to a changing pH environment. These characterizations of L‐lactate transport into isolated sarcolemmal vesicles and muscle strips revealed that both preparations yielded similar conclusions regarding the transmembrane movement of L‐lactate. By using this more physiological muscle preparation, contractile activity, induced by electrical stimulation, did not increase lactate uptake in skeletal muscle in the post‐exercise period whereas under similar conditions a marked increase in 2‐deoxy‐D‐glucose uptake occurred (+47%; P < 0.05). These data suggest that the transport of glucose and lactate in contracting muscle is regulated differently. These studies also show that the incubated muscle strip preparation may be useful for studying lactate transport in an intact cell system during physiological experiments.