Voltage‐dependent Ca2+ channels in Chinese hamster ovary (CHO) cells

Voltage‐dependent Ca2+ channels were identified in CHO‐K1 cells, currently used in molecular biology studies. Experimental data obtained at both macroscopic and single‐channel levels using the patch‐clamp technique show that the Ca2+ current in CHO cells is similar to the high‐threshold L‐type of Ca...

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Bibliographic Details
Published in:FEBS letters 1994-08, Vol.349 (2), p.289-294
Main Authors: Skryma, R., Prevarskaya, N., Vacher, P., Dufy, B.
Format: Article
Language:English
Subjects:
Animals
Barium - physiology
Calcium - physiology
Calcium channel
Calcium Channels - physiology
Calcium current
CHO Cells
CHO-cell
Cricetinae
Electric Conductivity
Electrophysiology
Potassium - physiology
Signal Transduction
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Summary:Voltage‐dependent Ca2+ channels were identified in CHO‐K1 cells, currently used in molecular biology studies. Experimental data obtained at both macroscopic and single‐channel levels using the patch‐clamp technique show that the Ca2+ current in CHO cells is similar to the high‐threshold L‐type of Ca2+ current previously observed in excitable cells. It can be carried by Ca2+ or Ba2+ ions, blocked by both inorganic (Co2+) and organic (nifedipine, isradipine) Ca2+ channel blockers. The unitary Ca2+ channel activity was characterized by a conductance of 19 pS in 60 mM Ca2+, by single exponential distribution of open times (t = 0.84 ms) and biexponential distribution of closed times (t f, = 1.67 ms, t s = 7.9 ms ). However, the functional role of these Ca2+ channels in CHO cells remains unclear.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(94)00690-3