The structure of the urokinase-type plasminogen activator receptor gene

The cellular receptor for urokinase-type plasminogen activator (uPAR) is a glycosylphosphatidylinositol (GPI)-anchored membrane protein that plays a central role in pericellular plasminogen activation. It contains 313 amino acid residues, including 28 cysteine residues in a pattern of three homologo...

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Bibliographic Details
Published in:Blood 1994-08, Vol.84 (4), p.1151-1156
Main Authors: CASEY, J. R, PETRANKA, J. G, KOTTRA, J, FLEENOR, D. E, ROSSE, W. F
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Blood coagulation. Blood cells
Coagulation factors
DNA Primers
Erabutoxins - genetics
Exons
Fundamental and applied biological sciences. Psychology
Genomic Library
Glycosylphosphatidylinositols - metabolism
HeLa Cells
Humans
Mice
Molecular and cellular biology
Molecular Sequence Data
Multigene Family
Polymerase Chain Reaction
Receptors, Cell Surface - genetics
Receptors, Urokinase Plasminogen Activator
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Snakes
Urokinase-Type Plasminogen Activator - metabolism
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Summary:The cellular receptor for urokinase-type plasminogen activator (uPAR) is a glycosylphosphatidylinositol (GPI)-anchored membrane protein that plays a central role in pericellular plasminogen activation. It contains 313 amino acid residues, including 28 cysteine residues in a pattern of three homologous repeats. The cysteine residue pattern suggests that uPAR belongs to a superfamily of proteins including CD59, murine Ly-6, and a variety of elapid snake venom toxins. A novel 1.7-kb uPAR cDNA was isolated that is missing exon 5 and that contains 380 bp not previously reported at the 5' end. This cDNA was used to probe a human genomic library from which three clones were isolated and analyzed. The uPAR gene consists of 7 exons spread over 23 kb of genomic DNA. Exons 2, 4, and 6 code for homologous domains within the mature protein, as do exons 3, 5, and 7; CD59-like homologous pairs are encoded by exons 2-3, 4-5, and 6-7, respectively. The structure of the gene for uPAR further confirms the relationship of this molecule to the superfamily containing CD59, Ly-6, and the elapid snake venom toxins.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.v84.4.1151.1151