Selection and comparison of Mycoplasma synoviae hemagglutinating and nonhemagglutinating variants

• Published in: Avian diseases 1985-10, Vol.29 (4), p.1170-1176
• Main Author: Rhoades, K.R
• Format: Article
• Language: English
• Subjects: AIR SACS; AIRSACCULITIS; Animals; Antibodies; ANTIGENIC RESPONSE; Antigens; DINDON; Erythrocytes; Erythrocytes - physiology; Hemadsorption; HEMAGGLUTINATION; Hemagglutination Tests; Hemagglutination, Viral; Lesions; MICOPLASMA; MYCOPLASMA; Mycoplasma - classification; Mycoplasma - pathogenicity; Mycoplasma Infections - pathology; Mycoplasma synoviae; MYCOPLASME; Optical density; PAVO; Poults; Pulmonary alveoli; SAC AERIEN; SACOS DE AIRE; Species Specificity; STRAINS; TURKEYS
• ISSN: 0005-2086; 1938-4351
• DOI: 10.2307/1590470

Hemagglutinating (HA) and nonhemagglutinating (NHA) variants were selected from each of two strains of Mycoplasma synoviae, WVU-1853 and Neb-3S. The HA titers of antigens prepared by 100-fold concentration of broth cultures were 1:2560 and 1:5120 for the HA variants and less than 1:5 for the NHA variants. Adsorption of erythrocytes to colonies of the variants was directly correlated with HA activity. The HA and NHA characteristics were stable in vitro, and there was no change in HA titers after repeated transfers in broth medium. Comparisons of pathogenicity indicated differences between strains but not between variants of each strain. Air-sac lesions resulting from exposure to variants of strain Neb-3S were marked, whereas those resulting from exposure to variants of WVU-1853 were slight. The HA titers of isolates recovered from turkey air sacs exposed to the Neb-3S variants varied considerably, suggesting in vivo instability of the HA characteristic. /// A partir de las cepas de Mycoplasma synoviae identificadas como WVU-1853 y Neb-3S, se seleccionaron variantes hemoaglutinantes (HA) y no hemoaglutinantes (NHA). Los títulos de hemoaglutinación de los antígenos preparados concentrando 100 veces los cultivos en caldo fueron 1:2560 y 1:5120 para las variantes HA mientras que para las variantes NHA fué menos de 1:5. La adsorción de eritocitos con las colonias tuvo una correlación directa con su actividad hemoaglutinante. La actividad HA o NHA se mantuvo estable in vitro aún después de pasajes repetidos en caldo. Estudios comparando la patogenicidad de las cepas indicaron que hubo diferencias entre las cepas pero no entre las variantes de cada cepa. Las variantes de la cepa Neb-3S produjeron lesiones notables en los sacos aéreos, mientras que la exposición con las variantes de la cepa WVU-1853 causaron lesiones leves. Los títulos de hemoaglutinación de las aislamientos obtenidos a partir de los sacos aéreos de pavos inoculados con variantes de la cepa Neb-3S variaron considerablemente, lo que sugiere una inestabilidad in vivo de las características hemoaglutinantes.