Chemotaxis, sporulation, and larvicide production in Bacillus sphaericus 2362 the influence of l-ethionine, and of aminophenylboronic acid

4-Aminophenylboronic acid (APBA), a known inhibitor of sporulation in Bacilli, as well as l-ethionine, a known inhibitor of chemotaxis in Enterobacteria, inhibited both sporulation and chemotactic behavior but not growth of Bacillus sphaericus. Both compounds also inhibited the methyl group turnover...

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Bibliographic Details
Published in:FEBS letters 1994-08, Vol.349 (3), p.416-419
Main Authors: Andreev, J., Dibrov, P.A., Klein, D., Braun, S.
Format: Article
Language:English
Subjects:
APBA, 4-aminophenylboronic acid
Bacillus - drug effects
Bacillus - physiology
Bacillus sphaericus
Bacterial Proteins
Bacterial Toxins - biosynthesis
Boronic acid
Boronic Acids - pharmacology
CFU, colony-forming units
Chemotaxis
Chemotaxis - drug effects
Ethionine
Ethionine - pharmacology
Inhibitor
MCP, Methyl-accepting chemotaxis protein
Membrane Proteins - antagonists & inhibitors
Methionine - metabolism
Methyl-accepting chemotaxis protein
Methyl-Accepting Chemotaxis Proteins
Methylation
Regulation
SAM, S-Adenosylmethionine, SDS-PAGE, Sodium dodecyl sulfate polyacrylamide gel electrophoresis
Spores, Bacterial - drug effects
Spores, Bacterial - physiology
Sporulation
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Summary:4-Aminophenylboronic acid (APBA), a known inhibitor of sporulation in Bacilli, as well as l-ethionine, a known inhibitor of chemotaxis in Enterobacteria, inhibited both sporulation and chemotactic behavior but not growth of Bacillus sphaericus. Both compounds also inhibited the methyl group turnover on the methyl-accepting chemotaxis protein (P53) in this microorganism. Sporulation of B. sphaericus was inhibited only when APBA was added to the growing culture before the late logarithmic stage. It was previously demonstrated that the ability of B. sphaericus to respond to chemoattractants sharply declines at the same age of the culture. Thus, it seems plausible that the action of both inhibitors upon sporulation may be attributed to the inhibition of some regulatory pathway common to chemotaxis and sporulation and involving protein methylation. Possible exchange of the nutrient depletion-related sensory information between chemotaxis and sporulation systems at the level of methyl group transfer is discussed.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(94)00714-4