Cloning and Expression of cDNA Coding for a New Allergen from the House Dust Mite, Dermatophagoides farinae: Homology with Human Heat Shock Cognate Proteins in the Heat Shock Protein 70 Family

An immunoreactive clone, which shares no homology with the major allergens, Der f I and Der f II, was isolated by screening of a Dermatophagoides farinae cDNA library with rabbit antiserum raised against an extract of the house dust mite and sera from patients with mite allergy. The deduced amino ac...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1994-03, Vol.115 (3), p.435-440
Main Authors: Aki, Tsunehiro, Fujikawa, Akihiro, Wada, Takeshi, Jyo, Toshihiko, Shigeta, Seiko, Murooka, Yoshikatsu, Oka, Satoru, Ono, Kazuhisa
Format: Article
Language:English
Subjects:
Allergens - chemistry
Allergens - genetics
Allergens - immunology
Allergens - isolation & purification
Amino Acid Sequence
Animals
Antigens, Dermatophagoides
Arthropod Proteins
Base Sequence
cDNA
Cloning, Molecular
Dermatophagoides farinae
DNA, Complementary - chemistry
DNA, Complementary - genetics
Dust
heat shock protein
Heat-Shock Proteins - chemistry
Heat-Shock Proteins - genetics
Humans
Immunoblotting
mite allergen
Mites - immunology
Molecular Sequence Data
Proteins - chemistry
Proteins - genetics
Proteins - immunology
Proteins - isolation & purification
Rabbits
Recombinant Proteins - chemistry
Recombinant Proteins - genetics
Recombinant Proteins - immunology
Recombinant Proteins - isolation & purification
Sequence Alignment
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
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Summary:An immunoreactive clone, which shares no homology with the major allergens, Der f I and Der f II, was isolated by screening of a Dermatophagoides farinae cDNA library with rabbit antiserum raised against an extract of the house dust mite and sera from patients with mite allergy. The deduced amino acid sequence of the cDNA of the clone is significantly homologous, up to 65.5%, to the carboxyl-terminal region of the heat shock cognate protein (hsc) 71 in the heat shock protein (hsp) 70 family. A pool of the recombinant protein-specific IgE purified from mite-allergic sera recognized a 67 kDa protein on a blot of ATP-binding components partially purified from the mite body extract, while the antibody did not bind to the major allergens. We conclude that the recombinant protein, one of several important allergens, may be a protein in the heat shock protein 70 family from the house dust mite, D. farinae.
ISSN:0021-924X
DOI:10.1093/oxfordjournals.jbchem.a124356