A Monoclonal Antibody Reactive With Human Eosinophils

EO-1, an IgGI murine monoclonal antibody raised against human eosinophilic leukemia cells, reacts with eosinophils, basophils, platelets, and a few (2%) mononuclear cells but not with neutrophils. In the bone marrow, mature and immature eosinophils and basophils express the EO-1 antigen, whereas imm...

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Bibliographic Details
Published in:Blood 1986-01, Vol.67 (1), p.50-55
Main Authors: Saito, Hiroshi, Yamada, Kazuyoshi, Breard, Jacqueline, Yoshie, Osamu, Mathé, I Georges
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - immunology
Antigens - analysis
B-Lymphocytes - immunology
Basophils - immunology
Cell Line
Eosinophils - immunology
Fluorometry
Humans
Leukemia - immunology
Mice
Mice, Inbred BALB C
Rosette Formation
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Description
Summary:EO-1, an IgGI murine monoclonal antibody raised against human eosinophilic leukemia cells, reacts with eosinophils, basophils, platelets, and a few (2%) mononuclear cells but not with neutrophils. In the bone marrow, mature and immature eosinophils and basophils express the EO-1 antigen, whereas immature myeloid cells do not. The distribution of EO-1 antigen on leukemic cells is concordant with this finding, ie, typical myeloid lines (HL-60, KG-1, and ML-1) and fresh acute myelogenous leukemia cells are all unreactive with EO-1. Immunoprecipitation of an extract from surface-131l-labeled platelets with EO-1 and sodium dodecyl sulfate polyacrylamide gel electrophoresis, under reducing or nonreducing conditions, yielded a specific band of molecular weight 23,000. Previously described mono-clonal antibodies reacting with eosinophils also recognize neutrophils. EO-1 is a unique antibody with specificity restricted to eosinophils, basophils, and platelets and might therefore be a valuable reagent for the study of their function and differentiation.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V67.1.50.50