Prostacyclin Stimulation of the Activation of Blood Coagulation Factor X by Platelets

• Published in: Science (American Association for the Advancement of Science) 1986-01, Vol.231 (4736), p.385-388
• Main Authors: Dutta-Roy, Asim K., Ray, Tarun K., Sinha, Asru K.
• Format: Article
• Language: English
• Subjects: Adenosine monophosphate; Adenylic acid; Adenylyl Cyclases - metabolism; Aggregation; Alprostadil - pharmacology; Animals; Biochemistry; Biological and medical sciences; Blood; Blood coagulation; Blood coagulation factors; Blood coagulation. Blood cells; Blood platelets; Blood Platelets - drug effects; Blood Platelets - metabolism; Blood Platelets - physiology; Cattle; Causes of; Cell aggregation; Centrifugation; Coagulation factors; Cyclic AMP - metabolism; Deoxyadenosines - analogs & derivatives; Deoxyadenosines - pharmacology; Dideoxyadenosine - analogs & derivatives; Epoprostenol - pharmacology; Factor X - physiology; Fundamental and applied biological sciences. Psychology; Humans; Hydrolysis; Molecular and cellular biology; Peptide Hydrolases - metabolism; Physiological aspects; Platelet aggregation; Platelets; Prostacyclin; Prostaglandin D2; Prostaglandins; Prostaglandins - pharmacology; Prostaglandins D - pharmacology; Thromboxanes; Ungulates; Zymogens
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.3001935

When platelets were incubated with prostacyclin, prostaglandin E$_{1}$, or prostaglandin D$_{2}$ at concentrations insufficient to increase the level of adenosine 3′,5′-monophosphate (cyclic AMP), coagulation factor X was activated by a platelet cysteine protease. Prostacyclin or prostaglandin E$_{1}$ at higher concentrations increased the cyclic AMP level and inhibited the activation of factor X by platelets. Inhibition of platelet adenylate cyclase by 2′,5′-dideoxyadenosine allowed the activation of the protease at higher concentrations of the autocoids. Prostaglandins A$_{1}$, A$_{2}$, B$_{1}$, B$_{2}$, E$_{2}$, F$_{2\alpha}$, 6-ketoprostaglandin F$_{1\alpha}$, and thromboxane B$_{2}$, which do not affect platelet cyclic AMP level, did not stimulate the protease.