Characterization of two novel filamentous phages of Xanthomonas

• Published in: Journal of general virology 1994-09, Vol.75 (9), p.2543-2547
• Main Authors: Lin, Nien-Tsung, You, Bih-Yuh, Huang, Chang-Yi, Kuo, Chung-Wen, Wen, Fu-Shyan, Yang, Jui-Sen, Tseng, Yi-Hsiung
• Format: Article
• Language: English
• Subjects: Bacteriophages - isolation & purification; Bacteriophages - physiology; Bacteriophages - ultrastructure; Biological and medical sciences; DNA, Viral - analysis; DNA, Viral - metabolism; Fundamental and applied biological sciences. Psychology; Immune Sera; Microbiology; Microscopy, Electron; Open Reading Frames; Restriction Mapping; Sequence Homology, Nucleic Acid; Systematics; Virology; Virus Replication; Xanthomonas; Xanthomonas campestris; Xanthomonas campestris vesicatoria
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-75-9-2543

1 Department of Botany, 2 Institute of Molecular Biology and 3 Department of Horticulture, National Chung Hsing University, Taichung 402, Taiwan, Republic of China Two filamentous phages of Xanthomonas campestris pv. vesicatoria and Xanthomonas oryzae pv. oryzae were isolated and designated øXv and øXo, respectively. They were similar to other filamentous phages of Xanthomonas in (i) shape, (ii) restrictive host specificity, (iii) high stability, (iv) an ssDNA genome, (v) a dsDNA as the replicative form (RF), (vi) propagation without lysis of host cells and (vii) ability to integrate into the host chromosome. These phages showed sequence homology to filamentous phage øLf of X. c. pv. campestris . øXv was inactivated by antisera against øXv, øXo and øLf, whereas øXo and øLf were inactivated only by their respective antisera and the anti-øXv serum. Both the single-stranded phage DNAs and the RF DNAs of øXv, øXo and øLf were able to transfect X. c. pv. vesicatoria, X. o. pv. oryzae and X. c. pv. campestris . Physical maps of øXv and øXo were constructed for the RF DNAs. Genome sizes were estimated, based on mapping data, to be 6.8 kb for øXv and 7.6 kb for øXo, larger than that of the øLf genome (6.0 kb). The difference in genome sizes appeared to result from insertions of large DNA fragments. These fragments and the regions mediating integration were localized in the physical maps. Present address: Pesticide Toxicology Department, Taiwan Agricultural Chemicals and Toxic Substances Research Institute, Wufeng, Taichung 413, Taiwan, Republic of China. Received 29 November 1993; accepted 11 April 1994.