Immunocytochemical localization of parathormone in the mammalian parathyroid gland using the protein A-gold technique

Electron-microscopic immunocytochemistry for the demonstration of parathormone in parathyroid chief cells was performed in adult male rats, gerbils, mice, and dogs, using the protein A-gold technique. Protein A-gold particles were detected over both large and small secretory granules in all the anim...

Full description

Saved in:
Bibliographic Details
Published in:Cell and tissue research 1986, Vol.243 (1), p.3-7
Main Authors: INOUE, Y, SETOGUTI, T
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cytoplasmic Granules - analysis
Cytoplasmic Granules - ultrastructure
Dogs
Fundamental and applied biological sciences. Psychology
Gerbillinae
Gold
Hormones. Regulation
Immune Sera
Male
Mice
Mice, Inbred Strains
Microscopy, Electron
Parathyroid Glands - analysis
Parathyroid Glands - ultrastructure
Parathyroid Hormone - analysis
Rats
Rats, Inbred Strains
Species Specificity
Staining and Labeling - methods
Staphylococcal Protein A
Thyroid. Parathyroid. Ultimobranchial body
Vertebrates: endocrinology
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Electron-microscopic immunocytochemistry for the demonstration of parathormone in parathyroid chief cells was performed in adult male rats, gerbils, mice, and dogs, using the protein A-gold technique. Protein A-gold particles were detected over both large and small secretory granules in all the animals examined. In the former, they were concentrated not only over type-I granules with a large core, but also over type-II granules with a small core. They were also located over atypical granules, including heterogeneously dense granules, granules having vesicles in a finely particulate core, and distorted granules. All labelled secretory granules were characterized by the presence of a clear halo of varying width around the core. Occasionally, Golgi cisternae as well as Golgi vacuoles with a finely particular content were also labelled. The labelling of the secretory granules was strong in dogs, moderate in rats and gerbils, and weak in mice. In addition, it was more intense in the non-osmicated preparations than in the osmicated preparations. The frequency of both types of large granules showed species differences. The possible factors involved in these differences are discussed.
ISSN:0302-766X
1432-0878
DOI:10.1007/BF00221846