Immunocytochemical localization of parathormone in the mammalian parathyroid gland using the protein A-gold technique

• Published in: Cell and tissue research 1986, Vol.243 (1), p.3-7
• Main Authors: INOUE, Y, SETOGUTI, T
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Cytoplasmic Granules - analysis; Cytoplasmic Granules - ultrastructure; Dogs; Fundamental and applied biological sciences. Psychology; Gerbillinae; Gold; Hormones. Regulation; Immune Sera; Male; Mice; Mice, Inbred Strains; Microscopy, Electron; Parathyroid Glands - analysis; Parathyroid Glands - ultrastructure; Parathyroid Hormone - analysis; Rats; Rats, Inbred Strains; Species Specificity; Staining and Labeling - methods; Staphylococcal Protein A; Thyroid. Parathyroid. Ultimobranchial body; Vertebrates: endocrinology
• ISSN: 0302-766X; 1432-0878
• DOI: 10.1007/BF00221846

Electron-microscopic immunocytochemistry for the demonstration of parathormone in parathyroid chief cells was performed in adult male rats, gerbils, mice, and dogs, using the protein A-gold technique. Protein A-gold particles were detected over both large and small secretory granules in all the animals examined. In the former, they were concentrated not only over type-I granules with a large core, but also over type-II granules with a small core. They were also located over atypical granules, including heterogeneously dense granules, granules having vesicles in a finely particulate core, and distorted granules. All labelled secretory granules were characterized by the presence of a clear halo of varying width around the core. Occasionally, Golgi cisternae as well as Golgi vacuoles with a finely particular content were also labelled. The labelling of the secretory granules was strong in dogs, moderate in rats and gerbils, and weak in mice. In addition, it was more intense in the non-osmicated preparations than in the osmicated preparations. The frequency of both types of large granules showed species differences. The possible factors involved in these differences are discussed.