Translation in a wheat germ cell-free system of RNA from mitochondria of the normal and Texas male-sterile cytoplasms of maize (Zea mays L.)

• Published in: Current genetics 1994, Vol.25 (1), p.73-79
• Main Authors: Hack, E. (Iowa State Univ., Ames (USA). Dept. of Botany), Hendrick, C.A, Al-Janabi, S.M, Crane, V.C, Girton, L.E
• Format: Article
• Language: English
• Subjects: ARN; Biological and medical sciences; Cell-Free System; Classical genetics, quantitative genetics, hybrids; Cytoplasm - metabolism; ESTERILIDAD MASCULINA CITOPLASMICA; EXPERIMENTACION IN VITRO; EXPERIMENTATION IN VITRO; Fundamental and applied biological sciences. Psychology; GENE; GENES; Genetics of eukaryotes. Biological and molecular evolution; In-vitro translation; Infertility - genetics; Mitochondria; Mitochondria - metabolism; MITOCHONDRIE; MITOCONDRIA; Plant Proteins - biosynthesis; Plant Proteins - genetics; Protein Biosynthesis; Pteridophyta, spermatophyta; RNA - genetics; RNA, Mitochondrial; STERILITE MALE CYTOPLASMIQUE; T-urf13 gene; Texas cytoplasmic male sterility; Triticum; Vegetals; ZEA MAYS; Zea mays - genetics; Zea mays - metabolism
• ISSN: 0172-8083; 1432-0983
• DOI: 10.1007/BF00712971

RNA isolated from etiolated seedling shoot mitochondria of maize (Zea mays L.) with normal (N) or Texas male-sterile (T) cytoplasm stimulated the incorporation of (35S)-methionine into protein when added to a cell-free protein-synthesizing system from wheat germ. Discrete polypeptides with molecular masses of up to approximately 67 kDa were synthesized, and the pattern of bands was distinct from that obtained with total RNA. Products of translation of T-urf13 RNA were identified by immunoprecipitation, and of atpA, cox1, and cox2 RNA by hybrid arrest of translation by the cloned gene. Several polypeptides were differentially synthesized from N and T mitochondrial RNA; these differences were more extensive than those found when isolated, intact, N and T mitochondria are allowed to synthesize proteins.