Immunohistochemical and immunochemical characterization of a new endothelial cell-specific antigen

A new monoclonal antibody (moab BW 200) of IgG3 kappa-isotype was generated which recognizes an epitope located on an antigen molecule restricted to human neoplastic and non-neoplastic endothelial cells. The molecular weight of the antigen was determined using immunoprecipitation analysis followed b...

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Bibliographic Details
Published in:The journal of histochemistry and cytochemistry 1986-02, Vol.34 (2), p.209-214
Main Authors: Alles, J U, Bosslet, K
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - immunology
Antigens - analysis
Biological and medical sciences
Blood Vessels - immunology
Endothelium - immunology
Epitopes - analysis
Factor VIII - analysis
Factor VIII - immunology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Histocytochemistry
Humans
Immunochemistry
Molecular Weight
Tissue, organ and graft immunology
von Willebrand Factor
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Summary:A new monoclonal antibody (moab BW 200) of IgG3 kappa-isotype was generated which recognizes an epitope located on an antigen molecule restricted to human neoplastic and non-neoplastic endothelial cells. The molecular weight of the antigen was determined using immunoprecipitation analysis followed by SDS-PAGE. Despite its similar molecular weight to FVIII-RAG, the antigen detected by moab BW 200 was shown to be different from FVIII-RAG.
ISSN:0022-1554
1551-5044
DOI:10.1177/34.2.2418100