Viral DNA Sequences Detected in a Hamster Liposarcoma Induced by Bovine Papillomavirus Type 4

1 Department of Zoology, University of Edinburgh, Edinburgh EH9 3JT and 2 Department of Veterinary Pathology, University of Glasgow Veterinary School, Bearsden, Glasgow G61 1QH, U.K. Following intradermal inoculation of bovine papillomavirus type 4 (BPV-4) into a Syrian hamster, a liposarcoma develo...

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Bibliographic Details
Published in:Journal of general virology 1986-01, Vol.67 (1), p.187-190
Main Authors: Moar, M. H, Jarrett, W. F. H, O'Neil, B. W
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Bovine papillomavirus 1 - genetics
Bovine papillomavirus 1 - pathogenicity
Bovine papillomavirus 4
Carcinogenesis, carcinogens and anticarcinogens
Cattle
Cricetinae
DNA, Neoplasm - isolation & purification
DNA, Viral - genetics
DNA, Viral - isolation & purification
Liposarcoma - microbiology
Medical sciences
Mesocricetus
Molecular Weight
Nucleic Acid Hybridization
Papillomaviridae - genetics
Tumors
Viruses
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Summary:1 Department of Zoology, University of Edinburgh, Edinburgh EH9 3JT and 2 Department of Veterinary Pathology, University of Glasgow Veterinary School, Bearsden, Glasgow G61 1QH, U.K. Following intradermal inoculation of bovine papillomavirus type 4 (BPV-4) into a Syrian hamster, a liposarcoma developed at the inoculation site 20 months later. The DNA of this tumour contained multiple copies of the BPV-4 genome which existed in a free unintegrated state. Unintegrated viral DNA and viral DNA isolated from virus particles from bovine alimentary tract papillomas revealed identical cleavage patterns with CpG methylation-resistant and -sensitive restriction enzymes: apparently there was no gross methylation of CpG sites in either case. The entire BPV-4 genome appeared to be represented in the tumour DNA. Keywords: papillomavirus, carcinogen, hamster liposarcoma Received 22 March 1985; accepted 24 September 1985.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-67-1-187