Isolation and characterization of two cDNAs encoding for compartment specific isoforms of O-acetylserine (thiol) lyase from Arabidopsis thaliana

cDNAs encoding for two isoforms of O-acetylserine (thiol) lyase (OAS-TL), which catalyzes the synthesis of cysteine, have been isolated from Arabidopsis thaliana. Secondary structure together with expression patterns derived during photomorphogenesis indicate cellular localizations in the cytosol an...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 1994-09, Vol.351 (2), p.257-262
Main Authors: Hell, Rüdiger, Bork, Christiane, Bogdanova, Natascha, Frolov, Igor, Hauschild, Rüdiger
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Arabidopsis - enzymology
Arabidopsis - genetics
Arabidopsis - radiation effects
Arabidopsis thaliana
Base Sequence
Cell Compartmentation
Cysteine Synthase - biosynthesis
Cysteine Synthase - genetics
Cysteine synthesis
Cytosol - enzymology
DNA, Complementary - genetics
Escherichia coli - genetics
Gene Expression Regulation
Genes, Plant - genetics
Genetic Complementation Test
IPTG, isopropyl-β-d-thiogalactoside
Isoenzymes - biosynthesis
Isoenzymes - genetics
Light
Molecular Sequence Data
O-Acetylserine (thiol) lyase
OAS-TL, O-acetylserine (thiol) lyase
Plastids - enzymology
Recombinant Proteins - biosynthesis
Restriction Mapping
Sequence Homology, Amino Acid
Space life sciences
Sulfate assimilation
Tissue Distribution
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:cDNAs encoding for two isoforms of O-acetylserine (thiol) lyase (OAS-TL), which catalyzes the synthesis of cysteine, have been isolated from Arabidopsis thaliana. Secondary structure together with expression patterns derived during photomorphogenesis indicate cellular localizations in the cytosol and plastids, thus allowing a direct comparison of compartment-specific forms within one species. The cytosolic OAS-TL complemented an E. coli auxotrophic mutant lacking cysteine synthesis. Both isoforms are represented by small gene families. They are expressed under all conditions investigated and were observed to increase in expression in plants grown with limited sulfate supply.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(94)00872-8