Age-dependent changes in proteins of Drosophila melanogaster

Several molecular theories of aging postulate that there are age-dependent changes in gene expression and that these changes contribute to the reduction in the viability of senescent cells. High-resolution, semiautomated, quantitative two-dimensional gel electrophoresis of many soluble proteins was...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1986-03, Vol.231 (4742), p.1157-1159
Main Authors: Fleming, J.E, Quattrocki, E, Latter, G, Miquel, J, Marcuson, R, Zuckerkandl, E, Bensch, K.G
Format: Article
Language:English
Subjects:
AGE
Age groups
AGING
Aging & longevity
ANALISIS
ANALYSE
ANALYSIS
Animals
Biochemistry
Biochemistry. Physiology. Immunology
Biological and medical sciences
Cell aging
Cell research
Cells
Cellular senescence
Cytological research
DROSOPHILA
Drosophila melanogaster
Drosophila melanogaster - metabolism
Drosophilidae
EDAD
Electrophoresis
ENVEJECIMIENTO
Fundamental and applied biological sciences. Psychology
Gels
GENE
Gene expression
GENES
Genetic heterogeneity
Genetics
Gerontology
Insecta
Invertebrates
Male
Molecular theory
Molecular Weight
Physiology. Development
PROTEINAS
PROTEINE
PROTEINS
Proteins - metabolism
Quantitative analysis
Space life sciences
VIEILLISSEMENT
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Summary:Several molecular theories of aging postulate that there are age-dependent changes in gene expression and that these changes contribute to the reduction in the viability of senescent cells. High-resolution, semiautomated, quantitative two-dimensional gel electrophoresis of many soluble proteins was used to test this hypothesis in Drosophila. Two-dimensional protein gel patterns were analyzed for each of three age groups of [$^{35}$S]methionine-labeled adult male Drosophila melanogaster, which, except for their spermatocytes, consist entirely of fixed postmitotic cells. Seven relatively abundant polypeptides expressed in middle-aged (28-day-old) flies were absent in both young (10-day-old) and old (44-day-old) flies. Quantitative analyses of an additional 100 polypeptides were carried out by computer-assisted microdensitometry of fluorograms of the gel preparations. These analyses revealed a significant age-related heterogeneity in the quantitative distribution of radiolabel in these proteins. The data indicate that the qualitative pattern of gene expression is identical in young and old flies, but that profound quantitative changes occur in the expression of proteins during the Drosophila life-span.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.3080809