Hemocyanin Respiratory Pigment in Bivalve Mollusks

Hemocyanins, high molecular weight oxygen-binding proteins, were identified in two species of protobranch bivalve mollusks, Acila castrensis and $\Upsilon $oldia limatula. Although hemocyanins have been reported in chitons, gastropods, and cephalopods, they have not been observed in the Class Bivalv...

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Published in:Science (American Association for the Advancement of Science) 1986-03, Vol.231 (4743), p.1302-1304
Main Authors: Morse, M. Patricia, Meyhöfer, Edgar, Otto, Joann J., Kuzirian, Alan M.
Format: Article
Language:English
Subjects:
Acila castrensis
analysis
Animal organs
Animals
Aquatic life
Biological and medical sciences
Biological Evolution
Bivalvia
Cylinders
Electron micrographs
Electrophoresis
Electrophoresis, Polyacrylamide Gel
Evolution
Fundamental and applied biological sciences. Psychology
Gels
Gills
hemocyanin
hemocyanins
Hemocyanins - isolation & purification
Hemocyanins - physiology
Hemolymph
Hemolymph - physiology
Invertebrates
Marine
Marine biology
melecular weight
Microscopy, Electron
Molecular weight
Mollusca
Mollusca - metabolism
Mollusca - physiology
Mollusks
Physiology. Development
Pigments
Proteins
Yoldia limatula
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description Hemocyanins, high molecular weight oxygen-binding proteins, were identified in two species of protobranch bivalve mollusks, Acila castrensis and $\Upsilon $oldia limatula. Although hemocyanins have been reported in chitons, gastropods, and cephalopods, they have not been observed in the Class Bivalvia. In A. castrensis the dissociation products of hemocyanin, characterized by gel electrophoresis, had a subunit molecular weight of approximately 250K. Negatively stained preparations of extracted hemocyanin formed protein aggregates in the shape of cylinders measuring 35 by 38 nanometers. X-ray microanalysis of hemocyanin aggregates in thin sections of $\Upsilon $. limatula demonstrated the presence of copper in the molecules. The discovery of hemocyanin in the protobranchs reinforces the primitive nature of the taxon and is further evidence that the major molluscan classes have a common ancestry.
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Patricia</creatorcontrib><creatorcontrib>Meyhöfer, Edgar</creatorcontrib><creatorcontrib>Otto, Joann J.</creatorcontrib><creatorcontrib>Kuzirian, Alan M.</creatorcontrib><title>Hemocyanin Respiratory Pigment in Bivalve Mollusks</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Hemocyanins, high molecular weight oxygen-binding proteins, were identified in two species of protobranch bivalve mollusks, Acila castrensis and $\Upsilon $oldia limatula. Although hemocyanins have been reported in chitons, gastropods, and cephalopods, they have not been observed in the Class Bivalvia. In A. castrensis the dissociation products of hemocyanin, characterized by gel electrophoresis, had a subunit molecular weight of approximately 250K. Negatively stained preparations of extracted hemocyanin formed protein aggregates in the shape of cylinders measuring 35 by 38 nanometers. 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Psychology</subject><subject>Gels</subject><subject>Gills</subject><subject>hemocyanin</subject><subject>hemocyanins</subject><subject>Hemocyanins - isolation &amp; purification</subject><subject>Hemocyanins - physiology</subject><subject>Hemolymph</subject><subject>Hemolymph - physiology</subject><subject>Invertebrates</subject><subject>Marine</subject><subject>Marine biology</subject><subject>melecular weight</subject><subject>Microscopy, Electron</subject><subject>Molecular weight</subject><subject>Mollusca</subject><subject>Mollusca - metabolism</subject><subject>Mollusca - physiology</subject><subject>Mollusks</subject><subject>Physiology. 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identifier ISSN: 0036-8075
ispartof Science (American Association for the Advancement of Science), 1986-03, Vol.231 (4743), p.1302-1304
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source American Association for the Advancement of Science
subjects Acila castrensis
analysis
Animal organs
Animals
Aquatic life
Biological and medical sciences
Biological Evolution
Bivalvia
Cylinders
Electron micrographs
Electrophoresis
Electrophoresis, Polyacrylamide Gel
Evolution
Fundamental and applied biological sciences. Psychology
Gels
Gills
hemocyanin
hemocyanins
Hemocyanins - isolation & purification
Hemocyanins - physiology
Hemolymph
Hemolymph - physiology
Invertebrates
Marine
Marine biology
melecular weight
Microscopy, Electron
Molecular weight
Mollusca
Mollusca - metabolism
Mollusca - physiology
Mollusks
Physiology. Development
Pigments
Proteins
Yoldia limatula
title Hemocyanin Respiratory Pigment in Bivalve Mollusks
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