Supplementary Listeria‐typing with defective Listeria phage particles (monocins)

The potential use of monocins for listeria typing was investigated. Monocins are defective phage particles still capable of lysing listerial cells. They were induced by u.v.‐irradiation, precipitated with polyethylene glycol and purified by density gradient centrifugation. Using 26 monocins, it was...

Full description

Saved in:
Bibliographic Details
Published in:Letters in applied microbiology 1994-08, Vol.19 (2), p.99-101
Main Authors: Zink, R., Loessner, M.J., Glas, Ingrid, Scherer, S.
Format: Article
Language:English
Subjects:
Bacteriological methods and techniques used in bacteriology
Bacteriology
Bacteriophage Typing - methods
Bacteriophages
Biological and medical sciences
Biotechnology
Defective Viruses
Fundamental and applied biological sciences. Psychology
Listeria
Listeria - classification
Microbiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The potential use of monocins for listeria typing was investigated. Monocins are defective phage particles still capable of lysing listerial cells. They were induced by u.v.‐irradiation, precipitated with polyethylene glycol and purified by density gradient centrifugation. Using 26 monocins, it was possible to type 48% of Listeria monocytogenes strains, 92% of L. innocua strains and 94% of L. ivanovii strains. Overall typability of 480 strains was 68%. None of the monocins was able to lyse L. grayi. Monocin typing was found to be a valuable supplementary tool for typing strains which were non‐typable by the Weihenstephan phage typing set. A combination of phage typing and monocin typing increased overall typability of Listeria strains to 95%.
ISSN:0266-8254
1472-765X
DOI:10.1111/j.1472-765X.1994.tb00915.x