In Situ Quantitation of Lymph Node Helper, Suppressor, and Cytotoxic T Cell Subsets in AIDS

We have used the novel monoclonal antibodies 9.3 and anti-Leu-8 in conjunction with other T cell markers to quantify T cell subpopulations in the paracortex, mantle, and germinal center compartments of frozen sections of lymph nodes from seven homosexual men with acquired immunodeficiency syndrome (...

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Bibliographic Details
Published in:Blood 1986-03, Vol.67 (3), p.596-603
Main Authors: Wood, Gary S., Burns, Bruce F., Dorfman, Ronald F., Warnke, Roger A.
Format: Article
Language:English
Subjects:
Acquired Immunodeficiency Syndrome - immunology
Adult
AIDS/HIV
Antigens, Differentiation, T-Lymphocyte
Antigens, Surface - analysis
Biological and medical sciences
Homosexuality
Humans
Immunodeficiencies
Immunodeficiencies. Immunoglobulinopathies
Immunopathology
Lymph Nodes - immunology
Lymph Nodes - pathology
Lymphatic Diseases - immunology
Male
Medical sciences
Middle Aged
Phenotype
T-Lymphocytes - classification
T-Lymphocytes - immunology
T-Lymphocytes, Cytotoxic - classification
T-Lymphocytes, Helper-Inducer - immunology
T-Lymphocytes, Regulatory - immunology
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Summary:We have used the novel monoclonal antibodies 9.3 and anti-Leu-8 in conjunction with other T cell markers to quantify T cell subpopulations in the paracortex, mantle, and germinal center compartments of frozen sections of lymph nodes from seven homosexual men with acquired immunodeficiency syndrome (AIDS) and five heterosexual controls. Antibody 9.3 allows dissection of the Leu-2+ cytotoxic/suppressor subset (Tcs) into 9.3+ cytotoxic cells (Tc) and 9.3- suppressor cells (Ts). Anti-Leu-8 allows dissection of the Leu-3+ helper subset (TH) into functionally distinct subpopulations. The data indicate that the T cells in patients with AIDS exhibit normal antigen expression but altered subset ratios. In this series, the data suggested that the reversal of the paracortical TH-Tcs ratio was due to an increase in T, with a concomitant decrease in TH and Tc. These changes were also reflected in a reversal of the normal paracortical Tc-Ts ratio (3.0) to
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V67.3.596.596