Cloning and functional expression of a neuronal calcium channel beta subunit from house fly (Musca domestica)

The primary structure of a calcium channel beta subunit (betaM) from housefly (Musca domestica) has been deduced by cDNA cloning and sequence analysis. The open reading frame encodes a 441-amino acid polypeptide with a calculated molecular mass of 48,755 Da. Whole-mount in situ hybridization indicat...

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Bibliographic Details
Published in:The Journal of biological chemistry 1994-09, Vol.269 (38), p.23668-23674
Main Authors: Grabner, M. (Universitat Innsbruck, Innsbruck, Austria.), Wang, Z, Mitterdorfer, J, Rosenthal, F, Charnet, P, Savchenko, A, Hering, S, Ren, D, Hall, L.M, Glossmann, H
Format: Article
Language:English
Subjects:
ADN
Alternative Splicing
Amino Acid Sequence
Animals
Base Sequence
CALCIO
CALCIUM
Calcium Channels - chemistry
Calcium Channels - drug effects
Calcium Channels - genetics
CATION
CATIONES
CLONACION
CLONAGE
Cloning, Molecular
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
Dihydropyridines - pharmacology
Diptera - genetics
DNA Primers - chemistry
Electric Conductivity
GENE
GENES
Larva
Molecular Sequence Data
MUSCA DOMESTICA
Nerve Tissue Proteins - genetics
PROCESOS DE TRANSPORTE EN EL SUELO
Recombinant Proteins - metabolism
SECUENCIA NUCLEICA
Sequence Homology, Amino Acid
SEQUENCE NUCLEIQUE
Solubility
TRANSPORT DANS LE SOL
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Summary:The primary structure of a calcium channel beta subunit (betaM) from housefly (Musca domestica) has been deduced by cDNA cloning and sequence analysis. The open reading frame encodes a 441-amino acid polypeptide with a calculated molecular mass of 48,755 Da. Whole-mount in situ hybridization indicates that betaM mRNA is predominantly expressed in neuronal tissues. Transcription of betaM mRNA is evident from stage 13/14 of embryogenesis up to adulthood. Different expression patterns of splice variants were found in larvae and in adult fly heads. Amino acid identity between betaM and mammalian beta subunits is lower (66-68%) than within mammalian beta subunits (74-80%). Calculation of a phylogenetic tree indicates that betaM is an ancestral form of the four distinct beta subunit gene products identified in mammalian tissues so far. Despite these sequence differences, betaM is able to enhance endogenous calcium channel activity in Xenopus laevis oocytes as well as dihydropyridine binding to membranes from COS 7 cells transfected with rabbit heart alpha 1 cDNA in the same manner as was previously shown for mammalian beta subunits
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(17)31567-3