Aqueous to cornea fluorescein distribution ratio in normal and swollen cornea

Intravitreal sodium fluorescein was used to simulate equilibrium fluorescein kinetics, thereby allowing simple measurement of the aqueous to cornea fluorescein distribution ratio. Two groups of rabbit corneas were studied: normal corneas and corneas wounded by freezing. The aqueous to cornea fluores...

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Bibliographic Details
Published in:Graefe's archive for clinical and experimental ophthalmology 1986-01, Vol.224 (2), p.156-159
Main Authors: MINKOWSKI, J. S, SAWA, M, BARTELS, S. P, KENYON, K. R, NEUFELD, A. H
Format: Article
Language:English
Subjects:
Animals
Aqueous Humor - metabolism
Biological and medical sciences
Cornea - anatomy & histology
Cornea - metabolism
Cornea - pathology
Corneal Diseases - metabolism
Corneal Diseases - pathology
Diseases of cornea, anterior segment and sclera
Edema - metabolism
Edema - pathology
Fluorescein
Fluoresceins - metabolism
Fluorometry
Medical sciences
Models, Biological
Ophthalmology
Photography
Rabbits
Reference Values
Spectrometry, Fluorescence
Tissue Distribution
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Summary:Intravitreal sodium fluorescein was used to simulate equilibrium fluorescein kinetics, thereby allowing simple measurement of the aqueous to cornea fluorescein distribution ratio. Two groups of rabbit corneas were studied: normal corneas and corneas wounded by freezing. The aqueous to cornea fluorescein distribution ratio was approximately 0.4, was not significantly different in groups of normal or wounded eyes and little variability was noted. In addition, a comparison of in vivo and in vitro measurements of corneal fluorescein concentration in wounded eyes suggests that in vivo protein-bound fluorescein in the cornea fluoresces less efficiently than free fluorescein.
ISSN:0721-832X
1435-702X
DOI:10.1007/BF02141490