Optical Biosensor for Monitoring Microbial Cells

The potential of a new optical biosensor, the resonant mirror, for detecting whole cells is demonstrated. Staphylococcus aureus (Cowan-1) cells, which express protein-A at their surface, were detected by binding to human immunoglobulin G (IgG) immobilized on an aminosilane-derivatized sensor surface...

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Published in:Analytical chemistry (Washington) 1994-08, Vol.66 (15), p.2465-2470
Main Authors: Watts, Helen J, Lowe, Christopher R, Pollard-Knight, Denise V
Format: Article
Language:English
Subjects:
Biochemistry
Biological and medical sciences
Biosensing Techniques
Biosensors
Biotechnology
Dextrans - chemistry
Fundamental and applied biological sciences. Psychology
Humans
Immunoglobulin G - metabolism
Methods. Procedures. Technologies
Silanes - chemistry
Staphylococcal Protein A - biosynthesis
Staphylococcus aureus - isolation & purification
Staphylococcus aureus - metabolism
Various methods and equipments
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container_issue 15
container_start_page 2465
container_title Analytical chemistry (Washington)
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creator Watts, Helen J
Lowe, Christopher R
Pollard-Knight, Denise V
description The potential of a new optical biosensor, the resonant mirror, for detecting whole cells is demonstrated. Staphylococcus aureus (Cowan-1) cells, which express protein-A at their surface, were detected by binding to human immunoglobulin G (IgG) immobilized on an aminosilane-derivatized sensor surface at concentrations in the range 8 x 10(6)-8 x 10(7) cells/mL. A control S. aureus strain (Wood-46), which does not express protein-A, gave no significant response. Immobilization of the capture ligand on aminosilane surfaces with and without a hydrogel coating of carboxymethyl-dextran was compared. The greatest binding response was observed with non-dextran-coated surfaces. The sensitivity of the technique was increased a 1000-fold by using a human IgG-colloidal gold conjugate (30 nm) in a sandwich assay format. S. aureus (Cowan-1) cells were detected in spiked milk samples at cell concentrations from 4 x 10(3)-1.6 x 10(6) cells/mL using the sandwich assay.
doi_str_mv 10.1021/ac00087a010
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source ACS CRKN Legacy Archives
subjects Biochemistry
Biological and medical sciences
Biosensing Techniques
Biosensors
Biotechnology
Dextrans - chemistry
Fundamental and applied biological sciences. Psychology
Humans
Immunoglobulin G - metabolism
Methods. Procedures. Technologies
Silanes - chemistry
Staphylococcal Protein A - biosynthesis
Staphylococcus aureus - isolation & purification
Staphylococcus aureus - metabolism
Various methods and equipments
title Optical Biosensor for Monitoring Microbial Cells
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