"Wired" Enzyme Electrodes for Amperometric Determination of Glucose or Lactate in the Presence of Interfering Substances

Glucose oxidase (GOX) or lactate oxidase (LOX) were immobilized in an osmium-based three-dimensional redox hydrogel that electrically connected the enzyme's redox centers to electrodes. The enzyme "wiring" hydrogel was formed by cross-linking poly(1-vinylimidazole) (PVI) complexed wit...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 1994-08, Vol.66 (15), p.2451-2457
Main Authors: Ohara, Timothy J, Rajagopalan, Ravi, Heller, Adam
Format: Article
Language:English
Subjects:
Animals
Biochemistry
Biological and medical sciences
Biosensing Techniques
Biosensors
Biotechnology
Blood
Blood Glucose - analysis
Calibration
Cattle
Enzymes
Enzymes, Immobilized
Fundamental and applied biological sciences. Psychology
Glucose - analysis
Glucose Oxidase - metabolism
Lactates - analysis
Lactates - blood
Lactic Acid
Methods. Procedures. Technologies
Mixed Function Oxygenases - metabolism
Oxidation-Reduction
Sugar
Various methods and equipments
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Summary:Glucose oxidase (GOX) or lactate oxidase (LOX) were immobilized in an osmium-based three-dimensional redox hydrogel that electrically connected the enzyme's redox centers to electrodes. The enzyme "wiring" hydrogel was formed by cross-linking poly(1-vinylimidazole) (PVI) complexed with Os-(4,4'-dimethylbpy)2Cl (termed PVI15-dmeOs) with poly(ethylene glycol) diglycidyl ether (peg). Glucose and lactate sensors exhibited typical limiting current densities of 250 and 500 microA/cm2, respectively. When the electrodes were poised at 200 mV (SCE), the currents resulting from electrooxidation of ascorbate, urate, acetaminophen, and L-cysteine were negligible. When a Nafion film was employed, the linear range was extended from 6 to 30 mM glucose and from 4 to 7 mM lactate. The redox potential of the gel-forming polymer was 95 mV (SCE). Glucose and lactate measurements performed in bovine calf serum correlated well with a substrate calibration in phosphate buffer.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac00087a008