Inhibition of growth factor-induced differentiation of PC12 cells by microinjection of antibody to ras p21

The protein products (p21) of the ras cellular proto-oncogenes are thought to transduce membrane signals necessary for the induction of cell division 1–4 . However, there is uncertainty as to the precise role of ras p21 in mediating ligand-membrane receptor signals leading to cell differentiation. T...

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Bibliographic Details
Published in:Nature (London) 1986-02, Vol.319 (6055), p.680-682
Main Authors: Hagag, Nabil, Halegoua, Simon, Viola, Michael
Format: Article
Language:English
Subjects:
Animals
Antibodies - immunology
Biological and medical sciences
Cell Differentiation - drug effects
Cell differentiation, maturation, development, hematopoiesis
Cell Line
Cell physiology
Cyclic AMP - physiology
Fundamental and applied biological sciences. Psychology
Humanities and Social Sciences
letter
Microinjections
Molecular and cellular biology
multidisciplinary
Nerve Growth Factors - pharmacology
Pheochromocytoma - pathology
Proto-Oncogene Proteins - immunology
Proto-Oncogene Proteins - physiology
Rats
Science
Science (multidisciplinary)
Time Factors
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Summary:The protein products (p21) of the ras cellular proto-oncogenes are thought to transduce membrane signals necessary for the induction of cell division 1–4 . However, there is uncertainty as to the precise role of ras p21 in mediating ligand-membrane receptor signals leading to cell differentiation. Treatment of rat phaeo-chromocytoma cells (PC12)with nerve growth factor (NGF) results in the induction of a number of phenotypic characteristics of sympathetic neurones, including cessation of cell division and outgrowth of neuronal processes (neurites). Here we report that microinjection of antibody to ras p21 into PC12 cells inhibited neurite formation and resulted in temporary regression of partially extended neurites, an effect which was observed up to 36 h after initiation of NGF treatment. Neurite formation induced by cyclic AMP was unaffected by injection of anti-p21 antibody. These results indicate that p21 is involved in the initiation phase of NGF-induced neurite formation in PC12 cells and has a role in hormone-mediated cellular responses distinct from cell proliferation.
ISSN:0028-0836
1476-4687
DOI:10.1038/319680a0