Cloning of cDNA encoding the murine IgG1 induction factor by a novel strategy using SP6 promoter

Complementary DNA encoding the IgG1 induction factor, the first lymphokine directed to B lymphocytes, from a murine T-cell line has been cloned using a new strategy. The putative primary amino-acid sequence was deduced from the nucleotide sequence determined. The lymphokine synthesized by the direct...

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Bibliographic Details
Published in:Nature (London) 1986-02, Vol.319 (6055), p.640-646
Main Authors: Noma, Yoshihiko, Sideras, Paschalis, Naito, Takayuki, Bergstedt-Lindquist, Susanne, Azuma, Chihiro, Severinson, Eva, Tanabe, Toshizumi, Kinashi, Tatsuo, Matsuda, Fumihiko, Yaoita, Yoshio, Honjo, Tasuku
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Analysis of the immune response. Humoral and cellular immunity
Animals
B-Lymphocytes - immunology
Base Sequence
Biological and medical sciences
Biotechnology
Chromosome Mapping
Cloning, Molecular
DNA - genetics
DNA Restriction Enzymes
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Genetic engineering
Genetic technics
Genetic Vectors
Growth Substances - genetics
Histocompatibility Antigens Class II - analysis
Humanities and Social Sciences
Immunobiology
Immunoglobulin G - biosynthesis
Interleukin-4
Lymphokines - genetics
Lymphokines, interleukins ( function, expression)
Methods. Procedures. Technologies
Mice
Molecular cloning
multidisciplinary
Promoter Regions, Genetic
Protein Biosynthesis
Regulatory factors and their cellular receptors
RNA, Messenger - genetics
Science
Science (multidisciplinary)
T-Lymphocytes - physiology
Xenopus laevis
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Summary:Complementary DNA encoding the IgG1 induction factor, the first lymphokine directed to B lymphocytes, from a murine T-cell line has been cloned using a new strategy. The putative primary amino-acid sequence was deduced from the nucleotide sequence determined. The lymphokine synthesized by the direction of this cloned cDNA has many other functions, such as production of B-cell growth factor-1 and induction of Ia on B cells.
ISSN:0028-0836
1476-4687
DOI:10.1038/319640a0