Expression of CMS-unique and flanking mitochondrial DNA sequences in Phaseolus vulgaris L

The expression of mitochondrial DNA sequences unique to a cytoplasmically male-sterile (CMS) line of Phaseolus vulgaris was investigated. RNA-blot hybridizations with strand-specific probes demonstrated CMS-unique transcripts (7.0, 6.8, 4.7, 3.3 and 2.8 kb) to be in the sense orientation with respec...

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Bibliographic Details
Published in:Current genetics 1994-03, Vol.25 (3), p.245-251
Main Author: Chase, C.D. (Florida Univ., Gainesville (USA). Inst. of Food and Agricultural Sciences. Horticultural Sciences Dept.)
Format: Article
Language:English
Subjects:
ADN
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
DNA, Mitochondrial - genetics
ESTERILIDAD MASCULINA CITOPLASMICA
Extrachromosomal Inheritance
Fabaceae - genetics
FERTILIDAD
FERTILITE
Fertility - genetics
Fertility restoration
Fundamental and applied biological sciences. Psychology
GENE
Gene Expression Regulation
GENES
Genes, Plant
GENETICA MITOCHONDRIAL
Genetics of eukaryotes. Biological and molecular evolution
GENETIQUE MITOCHONDRIALE
Mitochondria
Nucleic Acid Hybridization
Open Reading Frames
PHASEOLUS VULGARIS
Plant Diseases - genetics
Plant Proteins - genetics
Plants, Medicinal
Polyribosomes
Proton-Translocating ATPases - genetics
Pteridophyta, spermatophyta
RIBOSOMAS
RIBOSOME
RNA Splicing
RNA, Messenger - genetics
RNA, Messenger - metabolism
SECUENCIA NUCLEICA
SEQUENCE NUCLEIQUE
STERILITE MALE CYTOPLASMIQUE
Transcription
Transcription, Genetic
Vegetals
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Summary:The expression of mitochondrial DNA sequences unique to a cytoplasmically male-sterile (CMS) line of Phaseolus vulgaris was investigated. RNA-blot hybridizations with strand-specific probes demonstrated CMS-unique transcripts (7.0, 6.8, 4.7, 3.3 and 2.8 kb) to be in the sense orientation with respect to the longest open reading frames within the CMS-unique region. Hybridizations revealed co-transcription of CMS-unique and upstream, atpA-coding sequences to generate the 6.8 kb RNA. However, hybridizations with CMS-unique and flanking DNA probes accounted for only 4.9 kb of the longest and most abundant (7.0 kb) CMS-unique transcript, providing indirect evidence for the involvement of a splicing process in the generation of this transcript. Sedimentation experiments demonstrated the association of 7.0- and 6.8-kb CMS-unique transcripts with polyribosomes in seedlings and floral buds of a CMS line and a line restored to fertility by the nuclear gene Fr2. However, steady-state levels of the 7.0- and 6.8-kb transcripts were decreased in the restored line relative to the CMS line.
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00357169