Phosphorylation of a proline-directed kinase motif is responsible for structural changes in myogenin

Myogenin, a member of the MyoD family which governs skeletal muscle differentiation, was identified as a pair of phosphorylated bands on SDS-PAGE during myogenesis. The slow migrating form was found to be hyperphosphorylated myogenin. In vitro phosphorylation by CDC2 kinase caused a prominent reduct...

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Bibliographic Details
Published in:FEBS letters 1994-09, Vol.352 (2), p.236-242
Main Authors: Hashimotoa, Naohiro, Ogashiwa, Masayo, Okumura, Eiichi, Endo, Takeshi, Iwashita, Shintaro, Kishimoto, Takeo
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
CDC2 kinase
Cell Differentiation
Cell Line
Cyclin-dependent kinase
Gene Expression
Molecular Sequence Data
Muscle, Skeletal - cytology
Muscle, Skeletal - metabolism
Mutation - physiology
Myogenesis
Myogenin
Myogenin - genetics
Myogenin - metabolism
Peptide Fragments - analysis
Peptides - chemical synthesis
Phosphopeptides - analysis
Phosphorylation
Proline-Directed Protein Kinases
Protein-Serine-Threonine Kinases - metabolism
Serine - metabolism
Transfection
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Summary:Myogenin, a member of the MyoD family which governs skeletal muscle differentiation, was identified as a pair of phosphorylated bands on SDS-PAGE during myogenesis. The slow migrating form was found to be hyperphosphorylated myogenin. In vitro phosphorylation by CDC2 kinase caused a prominent reduction in electrophoretic mobility of myogenin. Furthermore, we demonstrated that phosphorylation of the serine residue at position 43 contributes to the modification of myogenin in vivo and in vitro resulting in the reduction in electrophoretic mobility. We propose here that a CDC2-like proline-directed kinase regulates myogenin activity through its phosphorylation.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(94)00964-3