Properties of a thermostable 4Fe‐ferredoxin from the hyperthermophilic bacterium Thermotoga maritima

A ferredoxin has been purified from one of the most ancient and the most thermophilic bacteria known, Thermotoga maritima, which grous up to 90°C. The reduced protein (Mr approx. 6300) contains a single S = 1 2 [4Fe 4S]1+ cluster with complete cysteinyl ligation, and was unaffected after incubation...

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Published in:FEMS microbiology letters 1994-08, Vol.121 (2), p.165-169
Main Authors: Blamey, Jenny M., Mukund, Swarnalatha, Adams, Michael W.W.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacteriology
Biological and medical sciences
Evolution
Fermentation
Ferredoxins - chemistry
Ferredoxins - isolation & purification
Fundamental and applied biological sciences. Psychology
Gram-Negative Anaerobic Bacteria - chemistry
Hyperthermophilic Archaea
Metabolism. Enzymes
Microbiology
Molecular Sequence Data
Thermostability
Thermotoga maritima
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container_title FEMS microbiology letters
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creator Blamey, Jenny M.
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description A ferredoxin has been purified from one of the most ancient and the most thermophilic bacteria known, Thermotoga maritima, which grous up to 90°C. The reduced protein (Mr approx. 6300) contains a single S = 1 2 [4Fe 4S]1+ cluster with complete cysteinyl ligation, and was unaffected after incubation at 95°C for 12 h. It functioned as an electron carrier for T. maritima pyruvate oxidoreductase. Remarkably, the properties and amino acid sequence of this hyperthermophilic bacterial protein are much more similar to those of ferredoxins from hyperthermophilic archaea, rather than ferredoxins from mesophilic and moderately thermophilic bacteria.
doi_str_mv 10.1111/j.1574-6968.1994.tb07094.x
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identifier ISSN: 0378-1097
ispartof FEMS microbiology letters, 1994-08, Vol.121 (2), p.165-169
issn 0378-1097
1574-6968
language eng
recordid cdi_proquest_miscellaneous_76744977
source Alma/SFX Local Collection
subjects Amino Acid Sequence
Bacteriology
Biological and medical sciences
Evolution
Fermentation
Ferredoxins - chemistry
Ferredoxins - isolation & purification
Fundamental and applied biological sciences. Psychology
Gram-Negative Anaerobic Bacteria - chemistry
Hyperthermophilic Archaea
Metabolism. Enzymes
Microbiology
Molecular Sequence Data
Thermostability
Thermotoga maritima
title Properties of a thermostable 4Fe‐ferredoxin from the hyperthermophilic bacterium Thermotoga maritima
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