The POX1 gene encoding peroxisomal acyl-CoA oxidase in Saccharomyces cerevisiae is under the control of multiple regulatory elements

Transcription of POX1, the gene encoding peroxisomal acyl-CoA oxidase in the yeast Saccharomyces cerevisiae, is controlled by the carbon source given for cell growth. The gene is repressed in glucose, derepressed in glycerol, and induced in oleate. This regulation is controlled by cis-acting element...

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Bibliographic Details
Published in:The Journal of biological chemistry 1994-09, Vol.269 (39), p.24480-24485
Main Authors: Wang, T. (Mount Sinai School of Medicine, New York, NY.), Luo, Y, Small, G.M
Format: Article
Language:English
Subjects:
Acyl-CoA Oxidase
Base Sequence
Coatomer Protein
DNA, Fungal
Fungal Proteins - genetics
Fungal Proteins - metabolism
GENE
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Fungal
GENES
Genes, Fungal
GENETICA
GENETIQUE
Microbodies - enzymology
Molecular Sequence Data
Oleic Acid
Oleic Acids - metabolism
ORGANITE CELLULAIRE
ORGANULOS CITOPLASMICOS
Oxidoreductases - genetics
OXIDORREDUCTASAS
OXYDOREDUCTASE
Promoter Regions, Genetic
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae Proteins
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Summary:Transcription of POX1, the gene encoding peroxisomal acyl-CoA oxidase in the yeast Saccharomyces cerevisiae, is controlled by the carbon source given for cell growth. The gene is repressed in glucose, derepressed in glycerol, and induced in oleate. This regulation is controlled by cis-acting elements in the 5' region of the gene, which bind regulatory proteins. By deletion analysis and DNA band shift assays, we have characterized three such elements in the POX1 upstream sequence, two upstream repression sequences and an upstream activating sequence. Each was able to regulate the transcription of a heterologous gene construct iso-1-cytochrome-c (CYC1)/lacZ
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(19)51109-7